Studies On The Chemical Constituents In Leaves Of Juglans Cathayensis Dode

Juglans Cathayensis Dode (Juglandaceae) grows in Shennongjia mountainous area of Hubei province of China. The leaves of the plant have been used as a folk medicine for the treatment of cancer. We found that the EtOAc-soluble fraction partitioned from the EtOH extract of Juglans Cathayensis Dode showed significant tumor inhibitory effects, which prompted us to initiate a chemical investigation of the EtOAc-soluble fraction of this plant. As a result, 19 com-pounds have been isolated from Juglans Cathayensis Dode and 16 of them are identified on the basis of the spectroscopic analysis and physical constants. The structures of them are Vanillic acid (hy-1), 2-Ethoxyjuglone (hy-2), Kaempfer-ol (hy-3), Kaempferol-3-O-glucopyranoside(hy-4), Quercetin-3-O-glucopyranoside (hy-5), Caffeic acid (hy-6), Phthalic acid (hy-7), 6,7-dihydroxycoumarin (hy-8), Nonacosyl alcohol (hy-9), Pentacosane alcohol (hy-10), Octadecanoicacid (hy-11), Hexadecanoic acid (hy-12),β-sitoterol (hy-13) and Daucosterol (hy-14). Except Octadecanoic acid, Hexadecanoic acid,Nonacosyl alcohol andβ-sitoterolthe remanent 12 compounds were isolated from the plant for the first time。T-he antitumor screening experiments on some compounds isolated from the lea-ves of Juglans Cathayensis reveals that hy-1-5, hy-7 and hy-8 have significant cytotoxicities ag-ainst the mice tumor cell S-180.