Studies On The Chemical Constituents And Anticancer Activity Of The Leaves Of Juglans Mandshurica Maxim

Juglans mandshurica Maxim distributes throughout urban and rural northeast of China. It distributes in Beijing, Hebei province, and Neimeng province also. A few distribute in Shanxi province, Russia, Korea,and Japan.Juglans mandshurica Maxim is one of the most important medical plants. But the reports about pharmacological activities and chemical components of Juglans mandshurica Maxim had not been reported one after another until the late 1980s.As the literature reported, the different spots of the Juglans mandshurica Maxims has the corresponding curative effects. Its immature fruit or the peel (black dragon clothes)has the obvious effects of analgesia, alleviate fever, relieve internal heat, stop the dysentery, and clear vision; Its kind of kernel may collect the lung to alleviate shortness of breath, warm the kidney and run intestines, which is used in the physique to be weak, the lung to empty cough, the kidney to empty lumbago, the constipation, the wet dream, the sexual impotence, to urinates the road stone, the milk lacks and so on; Its bark may alleviate fever and use in the bacillary dysentery, the bone tuberculosis, the grain of wheat swells and so on.At present, most study about Juglans mandshurica Maxim focuses on the root, skin and fruits, but even less concentrate on leaves. The research had indicated that Juglans mandshurica Maxim′leaves has the certain pharmacological activities. So it is quite significant to carry on the thorough system to the leaves′research. Based on the reviewing progress on studies of Juglans mandshurica Maxim′s pharmacological activities and chemical components, we gave futher research on the chemical components′withdrawals and separations, the structure appraisal and the biological activity. In the present thesis, we extracted and obtained the six chemical compounds from Juglans mandshurica Maxim using liquid-liquid extraction and column chromatography etc.We primarily identified the structure of 6 compounds according to theUV, IR, 1H-NMR ,13C-NMR ,MS,HMBC,HMQC, 1H-1H COSY datas. They are:quercetin(Ⅰ), 2′′E-quercetin-3-O-β-D-{6′′′′-O-[3′′-(4′′′-hydroxyphenyl)propenoyl]}glucopyranoside(Ⅱ), 1, 4, 8-trihydroxynaphthalene-O-β-D-[6′-O-(3′′, 4′′, 5′′-trihydroxybenzoyl)]glucopyranoside(Ⅲ), quercetin-3-O-β-D-glucopyranoside(Ⅳ), quercetin-3-O-β-D-galactopyranoside(Ⅴ)and octacosano(lⅥ).CompoundsⅡ,Ⅳ,Ⅴ,Ⅵare obtained from this plant first. CompoundⅠ,Ⅲare obtained from its leaves first.Finally we got the total flavone′s content of the EtOAc exract of Juglans mandshurica Maxim′leaves by triterpene。The experiment result shows that its total flavone content was approximately 82.6% on average.This result in order to give a basic data for the further research of the EtOAc exract of Juglans mandshurica Maxim′leaves.In addition, we gave pilot antineoplastic activity research on Juglans mandshurica Maxim′leaves. It showed that the EtOAc exract of Juglans mandshurica Maxim′leaves could control K562, A549, HT1080, MCF-7, HL-60, SGC, SWWC, A375.And it showed that its chroma and control percent were direct ratio.But it was more sensitive to K562 and HL-60 than to the other six.