The Effect Of Dietary Oil And Exogenous Cholate On Colon Tumorigenesis, Blood Glucose And Blood Lipids In Mice

Objective:To study the effect of Chinese traditional oils and exogenous cholate on feces pH,the contents of feces cholic acid,colon tumorigenesis,blood glucose and blood lipids.Methods:140 mice were randomly divided into 7 groups:Basic control(BC), Azoxymethane control(AC),Rapeseed oil(RO),Lard group(LG),cholate control group(CC),rapeseed oil and cholate(RC),lard and cholate(LC).At the beginning of the research,all mice were treated with four weekly ip ofAOM(10mg/kg.bw) except the mice of BC.Fresh feces pH was examined every two-week,feces cholic acid was examined every four-week.At the end of the research,mice were weighed and killed. The plasma triglyceride,cholesterol and glucose were examined.Colon tumors and the number of aberrant crypt foci(ACF) were detected.Results:1.Feces pH and cholic acid in feces:1.1 The feces pH in CC was significantly lower than that in AC,the cholic acid in feces in CC was significantly higher than that in AC(P＜0.05).1.2 The feces pH in RC was significantly lower than that in RO and CC,the cholic acid in feces in RC was significantly higher than that in RO(P＜0.05),no significant difference was found between RC and CC.1.3 The feces pH in LC was significantly lower than that in LG,and higher than that in CC,the cholic acid in feces in LC was significantly higher than that in LG (P＜0.05),no significant difference existed between LC and CC.1.4 The feces pH in LC was significantly higher than that in RG(P＜0.05),and no significant difference in cholic acid in feces was found between them.2.Colon tumorigenesis:2.1 No significant difference was found in the incidence of colon cancer and ACF between BC and AC,but the average number of ACF in each mouse in AC was significantly higher than that in BC(P＜0.05).2.2 There was no significant difference in the incidence of colon cancer and ACF between AC and CC,but the average number of ACF in each mouse in CC was significantly lower than that in AC(P＜0.05). 2.3 No significant difference was found in the incidence of colon cancer,ACF, and the average number of ACF in each mouse between RC and RO.So was the result between RC and CC.2.4 There was no significant difference in the incidence of colon cancer and ACF between LC and CC.The same result was observed between LC and LG.The average number of ACF in each mouse in LC was significantly higher than that in CC (P＜0.05),but no significant difference existed between LC and LG.2.5 No significant difference was found in the incidence of colon cancer,ACF, and the average number of ACF in each mouse between RC and LC.3.Blood lipids:3.1 The triglyceride in AC was significantly higher than that in BC(P＜0.05),no significant difference was found in the cholesterol.No difference was observed between male and female mice.3.2 The triglyceride in CC was significantly lower than that in AC,but the cholesterol was significantly higher than that in AC(P＜0.05).No difference was observed between male and female mice3.3 The triglyceride in RC was significantly lower than that in RO(P＜0.05),no difference existed in the cholesterol between them,and there was no difference between male and female mice.The triglyceride in male mice in RC was significantly lower than that in CC(P＜0.05),no difference existed in the cholesterol between them. The cholesterol in female mice in RC was significantly lower than that in CC (P＜0.05),no difference existed in the triglyceride between them.3.4 The triglyceride in LC was significantly lower than that in LG(P＜0.05),no difference existed between male and female mice.The cholesterol in male mice in LC was significantly higher than that in LG(P＜0.05),no difference existed in the cholesterol in female mice.There was no significant difference in triglyceride and cholesterol in male mice between LC and CC.The triglyceride in female mice in LC was significantly higher than that in CC,the cholesterol was significantly lower than that in CC(P＜0.05).3.5 The triglyceride and cholesterol in male mice in LC was significantly higher than that in RC(P＜0.05).The triglyceride in female mice in LC was significantly higher than that in RC(P＜0.05),but no difference existed in the cholesterol in female mice.4.Blood glucose:4.1 The blood glucose in AC was significantly higher than that in BC(P＜0.05), no difference existed between male and female mice..4.2 The blood glucose in CC was significantly lower than that in AC(P＜0.05), no difference existed between male and female mice..4.3 The blood glucose in RC was significantly lower than that in RO(P＜0.05), no difference existed between male and female mice.The blood glucose in male mice in RC was significantly lower than that in CC(P＜0.05),but there was no difference in female mice.4.4 No significantly difference existed in blood glucose between LC and LG, and no difference between male and female mice.The blood glucose in female mice in LC was significantly higher than that in CC(P＜0.05),but there was no difference in male mice.4.5 The blood glucose in LC was significantly higher than that in RC(P＜0.05), no difference existed between male and female mice.Conclusion:1.AOM is a specific colon carcinogen.The incidence of colon tumor in KM mice was very low,so this kind of mice is not sensitive to the AOM.2.AOM could increase plasma triglyceride and glucose,but didn't influence cholesterol.No other similar reports were found.3.Different dietary fat contents and fatty acid didn't affect the content of cholic acid in feces.4.Exogenous cholate could significantly increase the content of cholic acid in feces and reduce feces pH.5.Rapeseed oil could diminish the feces pH at the condition of cholate feed. The lard could enhance the feces pH at the condition of cholate feed.6.Exogenous cholate could significantly inhibit the formation of ACF.7.Rapeseed oil didn't change the incidence of colon cancer and the formation of ACF.8.The lard didn't affect the incidence of colon tumor,but could inhibit the formation of ACF.9.Exogenous cholate could significantly increase plasma cholesterol and decrease triglyceride.10.Rapeseed oil could reduce the triglyceride in male mice and the cholesterol in female mice.And it also has a trend of reducing the plasma triglyceride in female mice and the cholesterol in male mice.11.The lard could enhance plasma triglyceride and decrease cholesterol in female mice.The lard didn't influence plasma triglyceride and cholesterol in male mice.12.Exogenous cholate could significantly reduce blood glucose in mice.13.Rapeseed oil could decrease the blood glucose in male mice,and it also has a trend of reducing the blood glucose in female mice.14.The lard could promote the blood glucose in female mice,and it also has a trend of enhancing the blood glucose in female mice.