Study On The Relation Between Syncytiotrophoblast Microparticles And Pathogenesis Of Preeclampsia

ObjectivesUsing histology to study the morphological features of placenta in women with pre-eclampsia(PE) and using immunohistochemistry to study the expression of STBMs,TNF-αand Dysferlin in placentas obtained from women with PE in order to find their relations to the pathogenesis of PE.Methods:1 30 samples were selected randomly,including 10 women with normal pregnancy,10 women with mild PE and 10 women with severe PE.The morphological features of each groups was studied by hematoxylin-eosin (HE)staining.2 30 samples were selected randomly,including 10 women with normal pregnancy,10 women with mild PE and 10 women with severe PE.Using monoclonal antibody of TNF-αand CK7 to study the expression intensity of STBMs in placenta.And the number of STBMs was accounted in each groups. Using the monoclonal antibody of Dysferlin to detect the expression of Dysferlin in placenta in each groups. Results1 In normal placenta,the syncytiotrophoblast was localized in the superficial layer of the villus and its nuclei were arranged regularily.The cytotrophoblast was occasionally seen close to the syncytiotrophoblast.In preeclamptic placenta, the cytotrophoblast was proliferated,while the syncytiotrophoblast was in the state of aging.And the syncytial knots were frequently encountered.2 CK7 was mainly expressed in placental syncytiotrophoblast and it was positively stained in STBMs found in placental intervillous space.The number of the STBMs was significantly increased in the PE groups than that of the control group 1.86±0.29,and the severe PE group5.90±0.50 had more STBMs as compared to the mild PE group4.00±0.64.The expression intensity of TNF-αin placental tissue of the severe PE group was 29.87±8.17,in the mild PE group was 11.60±8.24,and in the control group was 11.49±7.33.It had significant difference between the the severe PE group and the mild PE group (P＜0.05).There was no difference between the mild PE group and the control group.The TNF-αwas positively stained in STBMs found in placental intervilous spaces.And its expression intensity was higher in the severe PE group as compared to that in the mild PE group and the control group.3 Dysferlin was expressed in each groups,and was exclusively located in the syncytiotrophoblasts and in the placental vascular endothelium.None of the other cell types in the placenta expressed detectable levels of dysferlin. Dysferlin was expressed in high levels at the apical plasma membrane of the syncytiotrophoblasts in severe PE group.The placental vascular endothelium showed a low level of expression and some were even absent of staining.The expression intensity of Dysferlin in placental tissue in the control group was 27.31±14.09,in the mild PE group was 39.64±17.29 and in the severe PE was 48.35±12.83.It had significant difference among the groups(P＜0.05).But no significant difference was found in endothelium among three groups. Conclution1 Because of the proliferation of cytotrophoblast and the aging of syncytiotrophoblast,the preeclamptic placenta could not maintain the normal construction and function,which ultimately resulted in ischemia and hypoxia of fetus.2 The STBMs found in placental intervillous spaces may play an important role in the pathogenesis of PE.They may be potential contributors to immunoreaction and the systemic inflammatory responsiveness in PE.The high expression intensity of TNF-αin the severe PE placenta indicate that this PE placenta is in a condition of serious hypoxia or ischemia.3 Dysferlin was positively expressed in placenta.Its staining intensity may reflect the severity of PE and its expression is related to the damage of placenta which induced by PE.