Expression Of Tumor Necrosis Factor-α And Cytokeratin 20 In The Progress Of Invasion And Metastasis In Colonic Cancer

Issue one Expression of tumor necrosis factor-αand cytokeratin 20 the process of invasion of colon cancerObjective: To investigate the expression and the relevance of tumor necrosis factor-α(TNF-α) mRNA and cytokeratin20(CK20) mRNA in the tissues of patients with colon cancer and the relation between the expression and the oncobiological behavior.Methods: Surgically resected and pathologically confirmed 30 cases of colon cancer patients were internalized, RT-PCR technique was used to detect the expression of TNF-αmRNA and CK20 mRNA in the tumor, para-tumor and incisal margin tissues.Resul: The positive rates of TNF-αmRNA expressions in the three groups were 70.0% and 43.3%, 20.0% respectively with a Statistical significant difference (P<0.01), and the positive rates of CK20 mRNA expression were 63.3%, 33.3% and 16.7% also with a Statistical significant difference (P<0.01), but there was no Statistical significant difference between the para-tumor and incisal margin groups (P>0.05). TNF-αmRNA and the CK20 mRNA expression were deeply correlated. TNF-αmRNA and CK20 mRNA was foreign to the differentiation of colon cancer (P>0.05), but TNF-αmRNA is closely related to colon cancer Dukes stages and the invasion extence in the intestinal wall (P<0.01).Conclusion: TNF-αmRNA can be used to speculate the invasional capacity of colon cancer as an objective indicator. Issue two Expression of TNF-αand CK20 in the lymphatic micro-metastasis in colon cancerObjective: To investigate the expressions of TNF-αmRNA and CK20 mRNA in the lymph nodes of patients with colon cancer and to discuss their relations to lymphatic micro-metastasis of colon cancer.Methods: A total of 398 lymph nodes were collected from surgically resected and pathologically confirmed 30 cases of colon cancer patients to be serially sliced and detected of lymphatic micro-metastasis as well as expression of TNF-αmRNA and CK20 mRNA through RT-PCR technique.Results: 20.2 percent of non-lymphatic node metastases excepted by conventional pathologic examination has been found lymph node metastases by serial sections examination. The result of expression of TNF-αmRNA and CK20 mRNA were consistent on the whole to that of serial sections examination and the three presented a close correlation.the expression of TNF-αmRNA and CK20 mRNA were increased positively to the Dukes stages (P<0.05). The expression TNF-αmRNA and CK20 mRNA in positively transfered lymph nodes was superior to that of negative ones (P<0.01).Conclusion: TNF-αmRNA and CK20 mRNA can accurately reflect lymphatic micro-metastases; uniform to CK20, TNF-αmRNA can also reflect the lymphatic micro-transfered capacity and probably plays an important role in the process of lymphatic micro-metastases of colon cancer.Issue three Impact of Shenqi Fuzheng injection to capacity of invasion of colon cancerObjective: To investigate the impact of Shenqi Fuzheng injection to the colon cancer cellular capacity of invasion and to expression of TNF-αmRNA, CK20 mRNA in colon cancer cell lines.Methods: Colon cancer cell lines Caco-2 and LS-174T were cultured in vitro. After adding different concentrations of Shenqi Fuzheng injection, RT-PCR method was used to detect TNF-αmRNA, CK20 mRNA the expression of control group as well as the three Shenqi Fuzheng injection groups of different concentration. Impact of different concentration of Shenqi Fuzheng injection to the ability of cell migration of was detected by Matrigel.Results: The expression of CK20 mRNA was no significant difference among the groups of different concentration of Shenqi Fuzheng injection. To the expression of TNF-αmRNA, the control group was significantly lower than that of drug groups and higher concentration group significantly lower than inferior concentration one (P<0.01).Conclusion: Shenqi Fuzheng injection has great influence on the ability of colon cancer cells'expression of TNF-αmRNA. Different concentration of Shenqi Fuzheng injection can inhibit the abilities of movement or invasion of colon cancer cells in different extents, the inhibit ability is gradually strengthened and the TNF-αmRNA expression is gradually weaker with the concentration increased.