Increased Distribution Of HPL In Stbms And RBCs Of Intervillous Space And Basal Plate Vein In Preeclampsia

Objections:This study detected the expression of HPL in STBMs of intervillous spaceand basal plate vein and increased adhesion status in the red blood cells surfaceof severe preeclampsia patients to investigate the correlation between the abovecondition and preeclampsia, and the protect mechanism of preeclampsia forbreast cancer.Materials and Methods：The expression levels of HPL in STBMs of intervillous space and basalplate vein are detected by GBI universal two-step immunohistochemistry andindirect immunofluorescence. Eighty women are divided into four groups,among which20women are belong to the group of severe onset preeclampsia,and20women are healthy pregnant women as the early control group, and20women are belong to the group of late onset preeclampsia, and20women arehealthy pregnant women as the late control group. We also carry a correlationanalysis between these expressions, adhesion status in the red blood cellssurface and HCT (Hematocrit). The expression levels of HPL-mRNA in basalplate are detected by RT-PCR.Results:1.The positive expression levels of HPL in STBMs of intervillous spaceand basal plate vein in the early onset severe preeclampsia group(21.55±1.89；6.92±0.46) are significantly higher than those in normal group（2.21±1.02；1.73±0.63）(P=0.000; P=0.000).The positive expression levels of HPL in STBMs of intervillous space andbasal plate vein in the late onset severe preeclampsia group(11.44±1.46； 4.35±0.41) are significantly higher than those in normal group（5.93±0.76；2.30±0.25）(P=0.003; P=0.017).The positive expression levels of HPL in STBMs of intervillous space andbasal plate vein in the early onset severe preeclampsia are significantly higherthan those in late onset severe preeclampsia (p=0.001; p=0.018)2. Adhesion incidence rates in the red blood cells surface of HPL are withone accord between intervillous space and basal plate vein：the group of earlyonset severe preeclampsia(90%) are obviously higher than those in normalgroup(5%), the group of late onset severe preeclampsia (55%) are obviouslyhigher than those in normal group (15%), and the group of early onset severepreeclampsia are obviously higher than those in late onset severe preeclampsiawhich have statistical difference.(X2=28.972, p=0.000; X2=7.033, p=0.013;X2=6.144, p=0.008)3. The surface adhesion HPL of red blood cells gather, and are adhered tothe vascular endothelial cell surface only in the group of early onsetpreeclampsia (50%) and late onset preeclampsia (10%).4. The HCT of the early onset severe preeclampsia patients aresignificantly higher than that of late onset severe preeclampsia and normalpregnant women.5. The positive expression levels of HPL in STBMs of intervillous spaceand basal plate vein are positively correlated with the patients’ HCT.（r=0.704,p=0.000; r=0.639, p=0.000）6. Adhesion incidence status in the red blood cells surface of HPL arepositively correlated with the patients’ HCT.(r=0.954, p=0.046)7. The expression levels of HPL-mRNA in basal plate are no significantdifferences between each group.Conclusion:1. Elevated expression levels of HPL in STBMs of intervillous space, can get through basal plate vein into the maternal circulation. And that might beone of the pathogens of preeclampsia.2. Assembled red blood cells result from expression levels of HPL inSTBMs of intervillous space, together with HPL are adhere to the vascularendothelial of pregnant women. And that might be one of the pathogens ofpreeclampsia.3. As the carrier of HPL, STBMs and red blood cells can facilitate thehyperplasia of breast gland bubble, which might be one of the reasons whywomen with PE bear low incidence of breast cancer.