Experimental Studies On Cidan Mixture Against Myocardial Ischemia

Salvia miltiorrhiza injection and acanthopanax senticosus injection are the Chinese medicine injection that commonly used to treat cardiovascular disease at present in clinical. In the last few years, salvia miltiorrhiza injection and acanthopanax senticosus injection were used together to treat anemic heart disease, and has obtained the better therapeutic effect, but, it still lack the dates which the animal experimentation studied on pharmacodynamics. This article is from animal experiments level, to carry out the Salvia miltiorrhiza injection and acanthopanax senticosus injection mixture (below to call that it CIDAN mixture) against myocardial ischemia role of the research, provides the basis for clinical medication, provides pharmacodynamics research foundation for the new medicine research and development.First of all, from the perspective of pharmacodynamics, used excised rat heart langendorff perfusion method, through the uniform design methods arrange experiment, filtering out the best CIDAN mixture of prescription composition. Used the best formula CIDAN mixture, carries on the animal experimentation according to 5 different dosage to group, to acanthopanax senticosus injection and Salvia miltiorrhiza injection alone as a control group, in addition supposes the model group and the sham-operation group. Observes each Group of ligation ramus descendens anterior arteriae coronariae sinistrae to fall a result the acute myocardial ischemia in rats when 5 min, 10min, 30min, 60min, 120 min, 240 min, 360 min the electrocardiogram change impacts, the index of serum enzymes and acute myocardial ischemia after 15 minutes when the rats heart hemodynamics status of the impact.The results showed that: the CIDAN mixture filtered out can obviously increase the isolated heart's coronary blood flow (P<0.01), the effect surpasses acanthopanax senticosus injection and Salvia miltiorrhiza injection single group(P<0.05),and can strengthen the cardiac muscle contraction amplitude(P<0.05).CIDAN mixture can significantly reduce aeteria coronaria ligation in rats result in acute myocardial ischemia electrocardiogram T wave elevation and the ST segment migration (P <0.05), its function can continue to amount to for 6 hours; Reduces the release of serum lactic dehydrogenase (LDH), the creatine kinase (CK) and the concentrations of oxidative products in serum malondialdehyde(MDA) of myocardial ischemia rats; CIDAN mixture can increase left ventricular systolic pressure (LVSP) and Left ventricular maximum pressure rate of change (±dp/dt_max), reduce Left ventricular end-diastolic pressure (LVEDP) of acute myocardial ischemia 15 min rats.The anti-myocardial ischemia animal experiments of CIDAN mixture show that its effect is better than acanthopanax senticosus injection and Salvia miltiorrhiza injection alone, It has the good prospect as a new drug research and development.