The Protective Effect And Mechanism Of Compound Acanthopanax Senticosus Injection On Myocardial Infarction In Rats

Coronary atherosclerotic heart disease is a kind of disease which refer to severe coronary artery atherosclerotic stenosis, occlusion, or spasm in the consolidated basis, and thrombosis caused by the coronary artery obstruction, which result in insufficient blood supply to coronary artery , myocardial ischemia or infarction . Coronary atherosclerotic heart disease is the most common cardical disease in China, Predilection to the elderly, the rate of ischemic heart disease has increased year by year in the past 20 years. Acute myocardial infarction is that A branch or of coronary artery refer to follicles due to arteriosclerosis and thrombosis which result in the corresponding part of the myocardial blood interrupt, leading to myocardial necrosis eventually. It is one of the most serious type in coronary heart disease Given the average life expectancy of our population was significantly longer, particularly the increase of coronary heart disease risk factors with the improved stands of our life, so the number of coronary heart disease will significantly increase, it has become the first killer which hurts human healthy in china. Therefore, coronary heart disease has become the focus of prevention and treatment of cardiovascular disease. Although drug treatments keep emerging, results of the prevention of coronary heart disease are still far from satisfactory. Thus, to search safe and effective drugs used for preventing from and curing of ischemic heart disease is an important task of medicine research.Compound acanthopanax senticosus injection is composed of Acanthopanax senticosus saponins and American ginseng PDS, 1:1 ratio between the two. Preliminary findings, The total saponins of Acanthopanax senticosus and Panax quinquefolium PDS showed significant effect of calcium channel blockers in a variety of animal models,Suggests that it may be an effective drug for treating arrhythmia .To this end, the research group will senticosus saponins and American Ginseng PDS compatibility of two active site developed into a 1:1 compound injection, to study the antiarrhythmic effect. Experts to identify scientific and technological achievements, Appraisal concluded that The results have been achieved international advanced level compared with other similar research. In veiw of acute myocardial ischemia is one of the major reasons leading to arrhythmia in clinical, a further reserch on the Protective Effect of Compound acanthopanax senticosus injection on Experimental Myocardial Infarction in Rats, studying the mechanism.In this experiment, the text was practiced by using the acute myocardial infarct(AMI) model of ligated left anterior descending coronary artery(LAD).MIS was calculated and activities of serum creatine hosphokinase(CPK), lactate Dehydrogenase(LDH), aspartate aminotransferase(AST) and superoxide dismutase(SOD), content of malondialdehyde(MDA), prostaglandin I2 synthase(PGI2) and thromboxane A2 (TXA2) content of blood serum were determined. Blood was collected to determine low specific viscosity, platelet aggeration rate and platelet adherence rate.30min after drug administration on the third day, copying acute myocardial infarct(AMI) model According to the literature method. after anesthetised with ether, Rats were fixed on the operating table in overhead position.Doing purse-string suture with 10 line. The chest was opened between the left third and fourth rib and the heart was exposed. After finding the Left anterior descending coronary artery, ligationing it at 2-3mm under the left atrial appendage Department with line 0 immediately. After the heart was return into the chest, the blood and water in the chest were squeezed out and close the chest swiftly. Entire Opening time of not more than the 30 seconds, the sham operation group only home line, but not ligation surgery. After 24 hours of ligating coronary artery, rats were anesthetized by 10% chloral hydrate (30mg/kg, ip). The hearts were taken out and perfused with NS through aorta. Atrial tissue and fat were removed, weighed, Left ventricular cross-section will be 4 to 5 and immersed in N-BT phosphate buffer in water bath at 37℃. Take them out after dying completely. Staining which normal tissue, ischemic tissue is not stained. ischemic myocardium is cutted and weighed. MIS is myocardial ischemia and left ventricular wet weight ratio. Activities of serum CK, LDH, AST and SOD, content of MDA, PGI2 and TXA2 of blood serum were determined. Blood was collected to determine whole blood viscosity, platelet aggregation rate and platelet adherence rate. The results showed that: CASI can be significantly reduced in rats with acute myocardial infarction 24h MIS, the activities of serum CK, AST and LDH declined. In addition, the content of serum MDA, TXA2 and viscosity of blood, platelet aggregation rate and platelet adherence rate were declined, activities of SOD,activities of GSH-Px and the content of PGI2 were increased markedly.Now that, the anti-ischemic drugs primarily by reducing myocardial oxygen consumption, Increaseing the oxygen supply to ischemic myocardium, improveing cardiac cell metabolism, preventing myocardial cell from damage of peroxidation and decreasing the blood viscosity of myocardial ischemia etc. CASI show a better pharmacological activity about several aspects as stated before, Prompted the protection of ischemic myocardium by combined result of a number of ways.