Effects Of Propofol On Platelet Activity And Coagulation Function And Blood Glucose In Rats With TypeⅡ Diabetes Mellitus

Objective: Platelet function is related to typeⅡdiabetes mellitus.Patients with typeⅡdiabetes mellitus undergoing opertiaon are increasingly growing. More and more anesthesiatists are concerned how to decrease the occurance of thrombopoiesis by using anesthesia techniques or drugs . So we tested the effects of continurous infusion of propofol on platelet activity and coagulation function and blood glucose in rats with typeⅡdiabetes mellitus and discussed it's possible mechanisiam.Methods: Thirty clearing inbreeding female SD rats were randomly divided into control group(CG),low-dose group(LG) and high-dose group(HG) (n=10 each).2% pentobarbital sodium was intraperitoneally injected for anesthesia before vena caudalis puncture to connect micro pump. 0.9% normal sodium with 12 mg·kg-1·h-1 were venously pumped in rats in CG. After intravenous injection of propofol with 5mg/kg, propofol was perfused with 3 mg·kg-1·h-1 and 6mg·kg-1·h-1 respectively for 30 minutes in LG and HG. Blood sample was collected from caudalis vein at 10 minutes before induction,30minutes after propofol continurous infusion and at 120 minutes after the end of infusion for testing the function of PAGmax,GMP-140,TXB2,blood glucose ,PT and APTT .The turbidimetry was used to measured the rate of platelet aggregation on the platelet aggregation device. Maximum intensity of aggregation was quantified as the maximum change in electric impedance for 10 min after the inducer was added.Three ml blood sample was mixed in 200μl EDTA anticoagulation plastics test tube,then was centrifugated at the rate of 3 500r/min at 4℃to evaluate TXB2 and GMP-140. Blood sample 1.8ml with 3.28% sodium citrate decoagulant to evaluate PAGmax. Blood sample 1.8ml with 3.28% sodium citrate decoagulant was evaluated PT and APTT. Blood sample 0.1ml was evaluated blood glucose.Results: There was no difference in weight(p>0.05). PAGmax,GMP-140 and TXB2 had no differences at T1 ,T2 and T3(p>0.05) in CG. PAGmax,GMP-140 and TXB2 in LG and HG at T2 were lower than those at T1 and T3(p<0.05).There was no difference of TXB2 between T1 and T3(p>0.05). In CG , there was no difference of TXB2 at T1 ,T2 ,T3(p>0.05).There was no difference of TXB2 at T1 and T3 between LG and HG (p>0.05). There was no difference of GMP-140 between T1 and T3 (p>0.05).In CG, There was no difference of GMP-140 at T1 ,T2 ,T3(p>0.05).There was no difference of GMP-140 at T1 and T3 between LG and HG(p>0.05).At T2, GMP-140 in HG was statistically lower than that in LG(p<0.05). There was no difference of PAGmax between T1 and T3 (p>0.05).In CG, There was no difference of PAGmax at T1 , T2 and T3(p>0.05).There was no difference of PAGmax between T1 and T3.At T2, PAGmax in HG was statistically lower than that in LG(p<0.05). In CG, there was no difference of blood glucose at T1 , T2 and T3(p>0.05).There was no difference of blood glucose between T1 and T3.At T2, blood glucose in HG was no difference of that in LG(p<0.05).There were no differences of PT and APTT at T1,T2 and T3 among three groups(p>0.05).Conclusion: Propofol could inhibit TXB2, PAGmax and GMP-140 and increase blood glucose.But propofol had no effect on PT and APTT .