Polymorphism Of The Two Y-STR Loci (DYS716&DYS721) In Chinese Han Population Of Shanxi

Objective The biomarker's polymorphism in Y-chromosome is lower than those in autosomes. More biomarkers are requested for further genetic analysis. We selected the candidate of Y-STRs loci (DYS716 & DYS721) from GDB Database, and investigated the new Y-STRs allelic frequencies and haplotype diversity in Chinese Han population of Shanxi. Their applicability in forensic casework were evaluated.Methods (1) EDTA-treated blood samples were collected from 114 healthy unrelated males in Chinese Han population of Shanxi. The blood of 20 females were collected as negative control. 20 families, different male animals and different tissues (including blood, muscle, et al) of one corpse were analyzed. (2)Genomic DNA was extracted via the method of phenol-chloroform. Primers sequences were synthesize according to GDB, and used to amplify the target DNA. (3)After PCR amplification, analyze the PCR products by non-denaturing polyacrylamide gel electrophoresis (PAGE) followed by silver-staining. (4)Calculate the forensic parameters.Result Five alleles are found in both DYS716 and DYS721 in Chinese Han population of Shanxi, which are named that DYS716*15,DYS716*16,DYS716*17,DYS716*18 and DYS716*19;DYS721*9,DYS721*10,DYS721*11,DYS721*12 and DYS721*13; The frequencies of DYS716 are 0.0263, 0.1754, 0.5263, 0.2456 and 0.0263, respectively. And the Gene Diversity (GD) value is 0.6361, both discrimination power (DP) and probability of exclusion (PE) are 0.6361 in forensic medicine; The frequencies of DYS721 are 0.0526, 0.5351, 0.2193, 0.1842 and 0.009, respectively. And the GD is 0.6344, both PD and PE are 0.6344 in forensic medicine; Haplotype diversity (HD) is 0.8810. No PCR products was observed in 20 female blood, and no mutation was found in 20 father-son pairs. The typing results of different tissues were identical. The two loci were specific to the human species.Conclusion The DYS716 and DYS721 loci are highly genetic polymorphism and stability. The data obtained can be used in individual identification, paternity test and population genetics investigation for Chinese Han population of Shanxi.