Xiaofutongjie Grain Catharsis Effect And Mechanism Of The Experimental Study

Objective: In the past pharmacodynamics of experiments and clinical studies have proved that Xiaofutongjie Grain can effectively on the basis of the treatment of constipation,Constipation of the pathological mechanism of Chinese medicine and Xiaofutongjie Grain of the legislative principle of prescription and compatibility characteristics.At the same time,Pai constipation mice by observing the time and the number of black fecal situation,To reflect the role of the drug under the strength of diarrhoea;By observing carbon black at the end of the small intestine constipation mice and rats from the advance of colon,On the drug to reflect the impact of intestinal smooth muscle,and thus judged on the role of rat intestinal movement.Constipation mice by measuring the water content of different Changduan to reflect the drug on rat intestinal increase in the water content,to determine the role of the main parts.Xiaofutongjie Grain observation of constipation guitar particles in plasma MTL,SS content changes,immune from the group to clarify the perspective of the drug on the mechanism of constipation.Methods:Academic exploring:Collect and sum up the history of literature related to constipation-related expositions,and in accordance with years of clinical trials,combined with modern medical research on the progress of constipation,Constipation of the Chinese etiology,pathogenesis,and Lifa Prescription Xiaofutongjie Grain of the legislative principle of particle characteristics and Compatibility.Experimental Study:1.Constipation mice defecation on time and the impact of grains: 60 mice were randomly divided into control group,model group,Maziren pill group,XFTJH, XFTJM,XFTJL,each group of 10.In addition to the control group,the rest of the group administered DC suspension replication model after the success of constipation,Model group and the control group given saline every day gavage,the group fed with the remaining 2% of the corresponding liquid ink.After administration,continuous observation of 8h,recorded the first row and mice have time,4h,8h mice stem from the faeces of grains, dry stool weight,defecation Dilute stool points and the number of animals. 2.Constipation mice on the movement of the small intestine:With the experimental group and an administration.At the end of 30min after administration,according to the mice 0.02ml/g at the end of the dose given carbon suspensionTo 20min after the mice were sacrificed from cervical measurement pylorus to the length of the ileum and ink from the pylorus to the forefront of distance,and the percentage of ink and attention to the small intestine in each group was observed whether the increased volume.3.Constipation rat colon driving force of the impact:Will be 60 rats do not give food but drinking water can,and 12h after anesthesia,In the womb line with a scalpel incision to do the plastic pipe from the ileocecal inserted colon,a depth of 1cm,Fixed ligation,Through the skin from the back muscles leads to prepare for administration.The next day will be 60 rats were divided into 6 groups,sub-modeling methods,the groups of drugs given in the first experiment with the same.The last time 30 min after the end of treatment,All the rats fed food containing 2% of ink to End liquid immediately after time,Another 60 by the feeding of non-liquid pigment indicator.Gavage immediately after light anesthesia,VSA incision along the lines do find ileocecal,Infusion of normal saline containing ink. In and around the ileum ileocecal ligation done with two-lane,cut in two between the lines and ileum time.Rats were sacrificed after 10 minutes,the total length of the colon and measurement ink promote distance,and the percentage of ink.4.Changduan constipation mice on the different absorption of water:Animals,both with the administration and the division of one.2h after administration to mice were sacrificed after mesenteric cut,with the bottom line since ligation Helicobacter pylori, intestinal cut;Further upside in the ileocecal ligation,cut intestine,and the removal of the small intestine.Ligation of the rectum to the end,cut intestine,large intestine removed. Weighing all the rats were small intestine and colon wet weight and recorded,and then the rat small intestine and colon,on the oven drying, and weighing out.5.Constipation in the plasma and intestinal tissue in MTL,the content of SS:Animals, both with the administration and the group of three.Plasma samples of each group collecting:After the treatment, the rats anesthesia, 2 ml femoral vein blood injected into the cylinder in the special blending, centrifugal 15 min,the plasma from 250μl,to be kept below -20℃seized.Each group intestinal tissue specimens Collecting:The rat femoral vein blood,and rapidly belly,in the duodenum and jejunum transfer of 0.5cm in length from the intestinal tissue and immediately frozen in liquid nitrogen.Measurement motilin sample processing:small intestine organizations to join the 1ml of acetic acid,and boil for 10min, homogenized,by adding PBS 1ml,ibid. Preservation methods.Somatostatin test sample processing:small intestine in boiled 9g/L NaCl in 1ml boiled for 3 min,cooling after acetic acid by adding 0.5ml of homogenate was made in the homogenate,and then 0.5ml sodium hydroxide solution and in,ibid.preservation methods.By RIA analysis of non-equilibrium method.Results:Academic exploring:Constipation disease in large intestine,spleen, stomach,lung incidence of disease is the major factor Qilin deficiency,and promote fatigue,loss of intestinal Run,stop the dregs for the constipation-pathological mechanism of the main Chinese medicine,treatment should be Yiqiyangyin,soft Kennedy Runzao,Wan under purge.Experimental Study:1.Constipation mice defecation on time and the impact of grains: Compared with model group,the control group,XFTJH, XFTJM, XFTJL and Maren Pills group could shorten constipation mice defecation first time,increase the weight and defecation defecation grains(P<0.05). And Maren Pills group,XFTJH There was a significant difference(P<0.05).2.Constipation mice on the movement of the small intestine:Compared with model group,XFTJH,constipation mice XFTJM small peristalsis and promote speed there was a significant difference(P<0.01) in a dose-dependent manner compared with Maren Pills, there has been a significant difference(P<0.05).3.Constipation rat colon driving force of the impact: Compared with model group, XFTJH,constipation XFTJM rat model of the large bowel movements and speed of advance there was a significant difference(P<0.01) compared with Maren Pills,XFTJH, XFTJM There was a significant difference(P<0.05).4.Changduan constipation mice on the different absorption of water:Compared with model group,XFTJH,XFTJM,XFTJL,Maren Pills group can make mice colorectal water content increased(P<0.05),and to XFTJH most significantly(P<0.01);XFTJH mouse small intestine so that water can be increase in the content,compared with the model group with statistical significance(P<0.05), while Maren Pills group to the small intestine does not affect the water content(P>0.05),compared with Maren Pills,XFTJH,the rat colon XFTJM Moisture content differences(P <0.05).5.Constipation in the plasma and intestinal tissue in MTL,the content of SS:Hey XFTJ in irrigation,plasma and intestinal tissue significantly increased in MTL,SS decreased significantly compared with the model group with statistical significance(P<0.05), especially in large doses the most significant(P<0.01),and Maren Pills Group,the only XFTJH was significant(P<0.05).Conclusion:XFTJ set of stringent,in view of machines,fill-combined with the same take-off and landing,were balanced,Yiqi Jianpi without mayonnaise greasy,Wan Jun attack intestinal catharsis without heat Runzao not Hanliang, effective for the treatment of constipation new agents.The drug can reduce constipation mice defecation time and increased defecation grains;constipation mice can increase intestinal movement,promoting the rate of increase in carbon Mexico;constipation can direct role in the rat large intestine, can be absorbed in the small intestine role in the large intestine,increase its role in promoting colorectal;increase constipation large intestine and small intestine of mice moisture content;constipation increase in plasma and intestinal tissue in MTL to reduce SS content,this is probably XFTJ increase intestinal motility and improve the symptoms of constipation mechanism.