The Expression And Significance Of Toll-like Receptor 9 In Cervical Intraepithelial Neoplasia And Cervical Cancer

Background:Malignant tumor is one of the most devastating diseases threatening human health nowadays,Cervical cancer is the most common malignant tumor in female reproductive system,ranking the second place in the female malignant tumor,is only inferior to breast cancer,Its mortality and morbidity is the highest among the female cancers in the developing countries.There are approximate 500000 new cases all over the world every year,it is about 138000 in our country, threatening women's life.Recently, with the development of cervical cancer screening,effectively enhance the detection rate of cervical cancer, many morning patients win the curing chance.However,the morbidity is more and more tending to the youth oriented, it's about forty percent patients are in reproductive age when final diagnosis. Although we unceasingly improve the treatment of cervical cancer,as the mainly treatment,operation is not only injured to patients,but also degrade quality of life.Regarding to progression patients,low exairesis rate and side effect of radiotherapy and Chem,it's hard to satisfied. Therefore, search the novel treatment is important for cervical cancer at present.The genesis and progress of tumor is correlated to immune system. Applied immunology principle to diagnosis,treat and prevent tumor is a hot spot of research.Toll-like receptors is a group of pattern recognition receptors belong to type I transmembrane protein , is an important immunity molecule.TLRs have been previously considered to be expressed only in immune cells,now there is increasing evidence that TLRs are expressed in non-immune cells as well. Some research indicate that TLRs participate many malignant tumor's genesis and progress,TLR9 is a important member of this family,whether relevant to the progression of cervical neoplasia is not clear.Objective:In this study, we undertook to detect TLR9 expression by immunohistochemical staining and western blot,in normal cervical tissue,cervical intraepithelial neoplasia and cervical cancer, approach the significance, in order to find out a new way for tumorous immunotherapy.Methods:1 The expression of TLR9 in 31cases of cervical cancer,12 cases of CINⅠ,15cases of CINⅡ-Ⅲand 11cases of normalcervical tissue were assessed by immunohistochemical staining.2 15cases of fresh cervical cancer and 10cases of normal cervical tissue were detected by western blot.3 Using SPSS13.0 software to analyze the data.Results:1 Immunohistochemical staining:TLR9 was buffy grains and mainly stain in tumor cell cytoplasmuse,utilized Image-Pro Pls 4.5 image analysis software processing the microscope images,using SPSS 13.0 analyze the data.The results suggested that TLR9 is weak expressed(36%)in normal cervical tissues,but it is expressed in almost all ISCC(29/31),and is strong in 38%.The expression is increased according to CINⅠ→CINⅡ-Ⅲ→ISCC (P<0.05),possess statistical significance,but we did not find the same result in FIGO stage,cell differentiation and lymph node metastasis.2 Western blot: the protein band of TLR9 was at 120KD,using UVP system to take picture and note the OD,the result was that TLR9 protein was obviously increased in cervical cancer compared to normal cervical tissue, (P<0.001), possessed statistical significance.Conclusion:1 TLR9 was not expressed or only weak expressed in the normal cervical tissue.2 The expression of TLR9 has positive correlation with the degree of cervical pathological change,CINⅠ→CINⅡ-Ⅲ→ISCC by turns increased, but the expression of TLR9 das not have correlation with FIGO stage,cell differentiation or lymph node metastasis.3 Immune response mediated by TLR9 participate the progression from cervical intraepiteial neoplasia to cervical cancer,may provide new immunotherapy for cervical cancer.