Meta-Analysis On The Association Of ApM1 Gene Single Nucleotide Polymorphisms And Type 2 Diabetes Mellitus In Chinese Population

Objective To evaluate the association of apM1 gene single nucleotide polymorphisms (SNPs) and Type 2 diabetes mellitus (T2DM) in Chinese population.Methods Odds ratios ( OR ) of apM1 gene SNPs gene/genotype distribution in patient with T2DM against healthy controls with normal glucose tolerance(NGT) were analyzed. Relative literatures were fully searched and screened. After data collection and gene loci selection, heterogeneity analysis was conducted. Sub-group analysis was performed to confirm the source of the heterogeneity according to the region of subject origin in the studies. The meta-analysis software(RevMan 4.3.1)was employed for summarizing all the studies, calculating the pooled OR and its 95%CI, and testing the overall effects. Egger's test and fail-safe number for P=0.05 (Nfs0.05) were performed for evaluating the publication bias. The sensitivity analysis by different effect model and sample size was employed for the reliability of meta-analysis. Results Nine literatures were obtained. ApM1 gene SNP45 showed remarkable heterogeneity among the studies (P<0.10). Sub-group analysis revealed that the discrepancy based on southern individuals was the main source of the total heterogeneity. The distribution frequency of apM1 gene SNP45G, SNP45GG, SNP276G and SNPGG was significantly higher in Chinese T2DM group than that in NGT group (P≤0.05). Their pooled OR and 95% CI were 1.50[1.12, 2.02], 2.15[1.53, 3.02], 1.23[1.03, 1.46] and 1.26[1.00,1.59], respectively. The distribution frequency of apM1 gene SNP45T, SNP45TT, SNP276T and SNPTT was significantly lower in Chinese T2DM group than that in NGT group (P≤0.05). Their pooled OR and 95% CI were 0.67[0.49, 0.90], 0.63[0.39, 0.99],0.79[0.66, 0.94] and 0.66[0.44,0.99, respectively. The distribution of apM1 gene SNP45TG and SNP276GT between T2DM and NGT group revealed no difference among these studies. The results of publication bias diagnostics and sensitivity analysis accounted for the reliability and stability of this Meta-analysis.Conclusions ApM1 gene SNPs are strongly associated with type 2 diabetes mellitus in Chinese population. The T→G mutation of SNP45 and the wild type G of SNP276 were risk factors for T2DM.