Severe Acute Pancreatitis MDA, SOD And Liver Microcirculation Changes And The Experimental Intervention Study Of Astragalus

Objective: Severe acute pancreatitis (severe acute pancreatitis, SAP) is a dangerous condition, complications more easily lead to multiple organ failure (MODS), treatment difficulties, the high mortality common surgical acute abdomen. The liver is the body's largest organ substantive glands, vascular extremely rich. Metabolism is the Centre as well as a major secretion, excretion, biotransformation and barriers organ, or a variety of synthetic clotting factor places. Many liver diseases accompanied significant changes in microcirculation, and the liver microcirculation metabolism in the liver is suitable for all types of material and the exchange of information important places, the changes in structure and function to a large extent affect liver function. In recent years the study: SAP liver microcirculation liver injury is a major factor. Pancreatitis at home and abroad on the liver microcirculation reported little change. Therefore, actively carrying out research in this area will be of great significance. Astragalus Air drugs for common with Yiqi solid form, and Water swelling role. A study has found that Astragalus role of free radicals, can improve myocardial energy metabolism, cell ultrastructure in particular the protection of mitochondrial structure, and increase intracellular SOD activity, due to reduced myocardial ischemia induced cell the accumulation of intracellular calcium and creatine kinase isoenzyme leakage; significantly decreased in the myocardial tissue MDA levels, and significantly improve the SOD and glutathione peroxidase live. Astragalus now mainly used for the treatment of cardiovascular diseases. Astragalus although it is reported for chronic pancreatitis, but there is no treatment for SAP reports. So this experiment retrograde cholangiopan creatography taken by the injection of 5% sodium taurocholate into rats SAP model. Observation of the pancreas and liver and general pathological changes of liver function: ALT, AST, TBLL serum amylase (AMY), lipase (LIP), serum malondialdehyde (MDA) content, superoxide dismutase (SOD) Activity changes of the liver were observed and the microcirculation of blood perfusion, capillary density and vascular diameter and after the intervention of the above indicators Astragalus changes. SAP, to investigate the mechanism of liver microcirculation, explore effective drug intervention, as a guide experimental basis for clinical treatment.Methods: Healthy male SD rats 72, weight 250±30g, were randomly divided into nine groups, each with eight, including: sham 2h, 6h, 12h (SO 2h, 6h, 12h), group SAP 2h, 6h, 12h(SAP 2h, 6h, 12h); Astragalus treatment interventions 2h, 6h, 12h (AM 2h, 6h, 12h); retrograde cholangiopancreatography adopted by the injection of 5% sodium taurocholate Preparation of SAP model, and the establishment via the tail vein route of administration, the treatment group by time and weight administration. By the time set for pancreas, liver general observation, measurement of ascites, collecting blood and tissue samples of liver histopathological changes in liver function Determination: ALT, AST, TBLL serum amylase (AMY), lipase (LIP), serum malondialdehyde (MDA) content, superoxide dismutase (SOD) activity changes of pancreatic pathology score. LISCA by laser-Doppler tissue perfusion images (LDPI) in vivo detection of liver microcirculation blood flow irrigation, WX-9B microscope observation of the liver microcirculation microcirculation. Blood perfusion of the liver, vascular wall itself changes: Traveling with microvascular distribution of the diameter size; intravascular the dynamic changes: blood velocity, blood flow pattern, red cell aggregation, leukocyte adhesion and change, whether white Micro thrombosis and embolism. SPSS11.5 experimental data using statistical analysis software.Results:1 Ascites: SAP Group have a wealth of the de facto red or dark red muddy ascites, in the ascites of between 3-12ml, and ascites with time extension significantly increased (P <0.05);2 pancreas and liver pathology morphological changes:2.1 general observation: SAP Group of the de facto existence of varying degrees of congestive pancreatic edema, hemorrhage and black flake necrosis, casual spot in the saponification; above gradually increased with time;2.2 optical microscopy: Pancreas: SAP group, the de facto different degrees of acinar and interstitial edema, hemorrhage and necrosis Flake, pancreatic pathology score significantly increased (P<0.01), liver: SAP phase of the rats SO phase and the corresponding group, liver significantly increased, the less the surface smooth, blunt edge of change, the color changes from red to dark brown, soft texture from stiffen. Varying degrees of liver cell edema, the District of infiltration of inflammatory cells and collagen connective tissue hyperplasia. And with time, the situation deteriorated.3 liver function ALT, AST, TBIL changes: SAP phase group and the corresponding phase SO group, ALT, AST, TBIL levels were significantly increased, there were significant differences (P <0.01).4 liver microcirculation changes: SAP phase group and the corresponding phase SO group, liver blood perfusion was significantly decreased microvessel diameter of the liver decreased blood flow velocity slower (P<0.01, P<0.05). Gradually increasing with time. SAP phase of the group that gradually increased the number of leukocytes and Yanbi rolling, a small amount of movement of tiny white and red blood cell aggregation thrombosis. SO phase of the group occasionally single WBC, no white-shuan, RBC aggregation phenomena and SAP group phase difference was significant (P<0.05).5 serum MDA, SOD changes: SAP phase group and the corresponding phase SO group, MDA levels were significantly increased (P<0.01), and with time and the trend was increasing. SOD levels were significantly lower (P<0.01), and with the extension of time was decreasing.6 AM intervention after the change:6.1 ascites: the time of ascites both decreased significantly (P <0.05);6.2 pancreas and liver pathology morphological changes:6.2.1 general observation: pancreatic edema, congestion, reduce the extent of necrosis, and peripancreatic fat capsule saponification in varying degrees to reduce liver increased not obvious, surface relatively smooth, soft texture;6.2.2 light microscope observation: Pancreas: Astragalus rats after the intervention point to edema and hemorrhage Flake mainly invasion of inflammatory cells to reduce the extent and necrosis narrow the scope, pancreatic pathology score decreased significantly (P<0.01) changes in liver pathology reduced liver cells edema, the District of infiltration of inflammatory cells and collagen connective tissue hyperplasia reduced significantly;6.3 liver function indicators ALT, AST, TBIL levels were significantly lower (P<0.05);6.4 microcirculation in the liver: blood perfusion of the liver increased, the liver increased microvessel diameter, flow speed, interleukin wall, tiny white and red blood cell aggregation thrombosis significantly reduced, there were significant differences (P<0.05);6.5 serum MDA, SOD changes: the group phase MDA levels were significantly lower (P<0.01), and with the extension of time was decreasing. SOD levels were significantly increased (P<0.01), and with time and the trend was increasing.Conclusion:1.5% sodium taurocholate retrograde cholangiopancreatography injection, the success of SAP SD rat model. Tail vein catheterization rehydration, this method is simple and reliable, reproducible, is a more ideal model of SAP preparation methods.2. SAP, the occurrence of liver damage and pathological changes of dysfunction, and increase with time and that SAP may be complicated by liver damage and pathological dysfunction3. SAP, the obstacles in the liver microcirculation, with its increased level of liver damage and increase the pathological changes, increase the degree of liver injury that led to a liver microcirculation and pathological changes in function of the damage.4. with SAP's progress, increased serum MDA, SOD activity declined, liver microcirculation increase, liver damage and increase the pathological changes, liver dysfunction increase that MDA, SOD is the microcirculation have an important obstacle to the media.5. after the intervention of astragalus, can reduce the SAP rat pancreas and liver pathological injury.6. after the intervention of astragalus, can significantly reduce the content of MDA, raising SOD activity, liver damage and reduce the pathological changes, liver function improved, so as to improve the liver microcirculation. Astragalus played a note of the liver to improve micro-circulation, reduce pathological liver damage, liver play a role in protection.