| Objective severe acute pancreatitis (SAP) rats were treated by the Chinese newmedicine Qing Yi grains (QYG). To observe the treatment effect, changes of serumamylase in animal models were observed. Pancreas, lung, intestine, liver, kidney wereharvested for pathological examination. Changes of serum peroxide dismutase (SOD)and malondialdehyde (MDA) were observed. Expression changes of nuclear factorκB (NF-κB) and high mobility group protein 1 (HMGB1) in different organs wereobserved to explore the possible treatment mechanism of Chinese new medicine QYGand provide experimental basis for clinical use.Methods Sprague-Dawley rats were randomly divided into sham operation group,SAP group and QYG group. Each group contained 18 rats and then randomly dividedinto 24h, 48h, 72h three groups, n=6. Rats were prepared by retrograde pancreaticduct injection of 5% sodium taurocholate (STC) (0.1ml/100g) to establish SAP model.QYG group received Qing Yi grains solution (1ml/100g) gavage treatmentimmediately after operation. Repeated administration was given every 12h. Animalsin each group were sacrificed at corresponding time points to collect the blood andorgans to detect serum amylase, T-SOD, MDA, HE staining for pancreas, lung,intestine, liver and kidney, S-P immunochemistry for expressions of organs NF-κBp65and HMGB1. Results1 Changes of the serum amylaseSerum amylase levels in SAP group and QYG group were significantly higherthan sham operation group. Compared with SAP group, serum amylase levels in QYG24h and 48h group were significantly decrease ((t =7.929,P=0.000；t =9.847,P=0.000)). They were no significant difference in 72h (t =0.769,P=0.460)2 Changes of serum SOD and MDASerum SOD levels in SAP group and QYG group were significantly lower thanthe sham operation group. At 48h and 72h after QYG treatment, serum SOD levelswere significantly higher than SAP group (t =-5.483,P=0.000；t =-4.519,P=0.001).There were no significant difference at 24h group (t =-0.747,P=0.472). Serum MDAin SAP group and QYG group were significantly higher than sham operation group ateach time point. Compared with SAP group, serum MDA levels of QYG group weresignificantly lower at each time point (t =7.128,P=0.000；t =4.544,P=0.001；t=5.501,P=0.001).3 Pathological changes in organsPathological changes occured in SAP rats'pancreas, lung, intestine, liver, kidney.QYG treatment can improve pathological changes in SAP rats'organs.4 Changes of NF-κB, HMGB1 expression in organsNF-κB, HMGB1 expression increased in SAP rats'pancreas, lung, intestine,liver, kidney. Organs NF-κB, HMGB1 expression decreased to different extent afterapplication of QYG treatment.ConclusioConclusion1 Serum amylase in SAP rats significantly increases. QYG can reduce high serumhyperamylasemia in SAP rats.2 In SAP rats, pancreas, lung, small intestine, liver and kidney showed damge invarying degrees. After application of QYG treatment, the extent of organ damageimproves. Traditional Chinese drug Qingyi grains can protect multiple organ damagein rats with acute pancreatitis. 3 In SAP rats, antioxidant capacity is decreased, resulting in a large number of oxygenfree radicals. QYG can increase the antioxidant capacity of body and reduce the levelof oxidative stress factors. Antioxidant effect may be one of the therapy mechanism ofQYG.4 NF-κB, HMGB1 expression increased in SAP rats'pancreas, lung, intestine, liver,kidney. Organs NF-κB, HMGB1 expression decreased to different extent afterapplication of QYG treatment. QYG can inhibit the organ NF-κB, HMGB1expression, and thus play its role in organ protection. |
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