The Effect Of Ping Yang Jiang Ya Fang On Blood Pressure And Insulin Resistance In Gan Yang Shang Kang Syndrome Model Of Spontaneous Hypertensive Rats

Objective:To observe the effect of Ping Yang Jiang Ya Fang on blood pressure and insulin resistance(IR)in Gan Yang Shang Kang Syndrome model of spontaneous hypertensive rats(SHR),and to explore its hypotensive effect and possible.underlying mechanisms.Method:Twenty-four SHR were randomly divided into four groups with 6 rats in each group,all of the SHR were drenched with Aconiti Praeparatae Decoction for four weeks to be made into Gan Yang Shang Kang Syndrome model.Another six healthy Wistar rats were taken as normal control group.From the fifth week,each SHR group was drenched with low or high dose of Ping Yang Jiang Ya Fang Decoction (8g/kg,16g/kg),the suspension of Nitrendipine(1.6mg/kg)or distilled water for thirty consective days,one time per day.After the first intragastric administration,the tail artery systolic pressure of all rats was measured in every two hours for twenty-four hours in order to observe the acute hypotensive effect of drugs.And it was also measured in every three days during drenching drugs in order to observe its chronic hypotensive effect.The changes of the fasting plasma glucose(FPG),fasting insulin(FINS),insulin sensitivity index(ISI),Resistin of all rats were also determined before and after therapy.Result:①.In the acute hypotensive experiment,low or high dose of Ping Yang Jiang Ya Fang Decoction and Nitrendipine can reduce the tail artery systolic pressure of SHR(P＜0.05),but there wasn't significant difference in twenty-four hours among them(P＞0.05).The changes of systolic pressure of SHR in the groups with low dose or high dose of Ping Yang Jiang Ya Fang Decoction were smaller than that with Nitrendipine during twenty-four hours(P＜0.05).In the chronic hypotensive experiment,they can also decrease the tail artery systolic pressure of SHR (P＜0.05).The hypotensive effect of high dose of Ping Yang Jiang Ya Fang Decoction was better than that of low dose(P＜0.05).②.The plasma FINS and resistin of SHR in the group of low or high dose of Ping Yang Jiang Ya Fang Decoction,or Nitrendipine significantly decreased (P＜0.05),while the ISI significantly increased(P＜0.05)after treatment. And the effect of either low or high dose of Ping Yang Jiang Ya Fang Decoction were better than that of Nitrendipine(P＜0.05).Conclusion:①.Ping Yang Jiang Ya Fang has obvious acute and chronic hypotensive effects to Gan Yang Shang Kang Syndrome model of SHR.②.Ping Yang Jiang Ya Fang can improve the IR of Gan Yang Shang Kang Syndrome model of SHR.