Studies On The Quality Standardization For Gentiana Scabra Bge.

Gentian is the roots of plants Gentiana manshurica Kitag.,Gentiana scabra Bge.,Gentiana triflora pall. or Gentiana rigescens Franch. of Gentianaceae. It has the efficacies of clearing heat and drying damp,discharging fire for liver and gall and damp-heat jaundice,hidden-arsine gargalesthesia, morbid leucorrhea, persistent erection, eczema pruritus, conjunctival congestion, deafness, hypochondriac pain, bitter taste, infantile convulsion for therapiesThis experiment is divided into six chapters altogether, including systemic comparison for ash contents, water contents, alcohol extractive contents, polysaccharide contents and gentiopicroside contents in gentian roots from different areas or harvesting in different times, studies on extraction technology for polysaccharide and essential oil from gentian roots, comparison and analysis of the pharmaceutical action of alcohol extractive in gentian roots from four different areas.Samples from 10 producing areas in 6 provinces altogether were collected for experiment analyzing. The samples were gentiana roots from Gentiana scabra Bge., Gentiana manshurica Kitag. The 10 different times Gentiana scabra Bge. Samples were collected from the beginning of September to the end of October in Zuo Jia in 2005.The ash contents in gentian roots from different areas or harvesting in different times achieve the pharmacopeia ordain cost. The water contents in gentian roots from different areas were from 5.3648% to 6.9401%, and 5.0488% to 9.6281% in different times. The alcohol extractive contents in gentian roots from different areas were from 15.3407% to 41.1780%, and 33.8837% to 40.8272% in different times.The chapter of study for gentian polysaccharide is to optimize and establish the extraction process of gentian polysaccharide. To extract gentian polysaccharide and determine contents in gentian roots from different areas or harvesting in different times. The ultrasonic wave extraction optimum process parameters are as fouons: temperature is 80℃, extract time is 20m, mass ratio of water is 8 times. The microwave-assisted extraction optimum process parameters are as fouons: wave power is 450 w, mass ratio of water is 14 times, extract time is 4m. The ultrasonic wave extraction is better than microwave-assisted extraction for gentian polysaccharide extraction, and they are both better than water decoction. The difference is marked of polysaccharide contents in different areas. The hightest polysaccharide content is 24.8747% in Tao Hua-tan of An Hui Pronvince, the lest is 4.6989% in Ji Xi of Hei Long-jiang Province. The contents are from 8.1516% to 24.5675% in different times. This study establish foundation for gentian polysaccharide extraction by industrialization, and afford material antecedent for further research into pharmacodynamic function of the polysaccharide in gentian roots.The chapter of study for gentian gentiopicroside is to determine the contents of gentiopicroside in gentian roots from different areas or harvesting in different times, according to method in pharmacopeiae, used VPODS(150mm×4.6mm, 0.25μm). To analysis by the data processing, found that the lest content is 0.5136% in Hou Ma of Shan Xi Province, don not achieve the pharmacopeia ordain cost. The highest content is 9.8945% in Zuo Jia of Ji Lin Province. The contents are from 3.3528% to 7.2726% in different times.The chapter of study for gentian essential oil is to establish the best extraction by SFE-CO₂. Use GC-MS to compare the essential oils extracted from Radix Gentianae by supercritical fluid extraction(SFE) and steam distillation(SD). The SFE-CO₂ optimum process parameters are as fouons: the temperatrue is 30℃, the garticle size is 20～40 mesh, the extraction compressive stress. There were 28 components from Radix Gentianae by SFE were separated and identified. There were 27 components from Radix Gentianae by SD were separated and identified. The SFE-CO₂ is better than the steam distillation.Establishment of experimental model for liver injury of CCl₄ in Wistar rats. Compare the therapies of alcohol extractive in gentian roots from four different areas. Result is that Zuo Jia is the best.This essay aim at ash contents, water contents, alcohol extractive contents, polysaccharide contents and gentiopicroside contents in gentian roots from different areas or harvesting in different times, and study on the extraction for polysaccharide and essential oil in gentian roots. All is to establish the good foundation for the quality standardization of gentian.