A Study On Relationship Between Expression Changes Of Chemokine CXCL16 In Peripheral Blood Mononuclear Cells And Acute Coronary Syndrome

Objective: In this study, we measured and compared express of chemokine CXCL16 in patients with Acute Coronary Syndrome peripheral blood mononuclear cells to investigate the effect of inflammation and the chemokine on the genesis and development of ACS and evaluate the value of CXCL16 in different angiographic morphology of coronary lesions in ACS and find out the association betweem CXCL16 expression and clinical types of ACS. To investigate the associativity between the expression level of CXCL16 on peripheral blood mononuclear cells and coronary artherosclerosis and investigate the difference of the expression level of CXCL16 on peripheral blood mononuclear cells in patient of AMI,UAP. To investigate the associativity between the expression level of CXCL16 on peripheral blood mononuclear cells and the numbers of branches conronary stenosis.Methods: 40 consecutive patients with ACS were diagnosed by coronary angiography in the hospitalized patients in the first hospital of Nanhua university. Each one had greater than or equal to 50% stenoses of greater than or equal to one coronary artery.The patients were divided into two groups : Acute myocardial infarction group (AMI) 20 cases; Unstable angina pectoris group (UAP) 20 cases. According to the number of suffered coronary artery, single branch, double branches and three/several branches were defined.Lymphocytes and monocyes were isolated by lymphocyte separation medium. The levels of CXCL16 mRNA and protein expression in monocyte and lymphocyte(PBMCs) coming from the subjects were tested by RT-PCR and western blot. Computer picture analyzing system was also used to measure the levels of CXCL16 mRNA and protein expression. Statistical analyses were performed using SPSS 13.0 for PC.Values were expressed as mean±standard deviation (±S) for continuous variables and number and percentage(%)for binary or polynary variables.Comparisons among different groups were made by a two-tailed Student test for continuous variables and by Pearson chi-square test and Fisher's exact test for binary variables. Difference were regarded as statistically significant when P values was <0.05.Result: The expression of CXCL16 were increased in ACS patients than in health volunteers(P<0.05).There was no significant difference in the expression of CXCL16 between UAP and AMI group(P>0.05). The numbers of branches conronary stenosis is no significantly different between UAP group and AMI group.The incidence of single-branch stenosis and double-branch and several-branch stenosis with two groups were no different grade stenosis (P>0.05).The expression of CXCL16 in AMI patients was no significantly positive correlated with the numbers of branches conronary stenosis (r=0.055,P=0.818).The expression of CXCL16 in UAP patients was no significantly positive correlated with the numbers of branches conronary stenosis (r=0.321, P=0.167).The expression of CXCL16 in ACS patients was no significantly positive correlated with the numbers of branches conronary stenosis (r=-0.127,P=0.435). The numbers of branches conronary stenosis was no significantly associated with the expression of CXCL16 respectively.Conclusion: The expression of CXCL16 in peripheral blood monocyte is higher in ACS patients. The inflammation reaction mediated by CXCL16 may participate in the pathogenesis of ACS.CXCL16 may take part in the the pathophysiological process of atheromotous plaque's break which lead to the genesis and development of ACS. So CXCL16 can be regarded as a novel marker of inflammation.But the expression of CXCL16 are not positive correlated with the numbers of branches conronary stenosis. The results demonstrated that there were activation of inflammatory cells and inflammation in patients with ACS, CXCL16 might play an important role in the disruption and thrombosis of plaque and the pathogenesis of ACS.