The Study Of The Expression Of CXCL16 And CXCR6 After The Acute Myocardial Infarction

Background: More and more study demonstrate that acute myocardial infarction is closely related to inflammation reaction, The role of autoimmune in the ventriclar remodeling process after myocardial infarction has called more and more attention. After myocardial infarction some structural protein released or many self-antigen exposured, thus autoimmune can be induced. Myosin is a exclusive cardial contractable protein, can induce the humoral immunity in the MI patient. it has verified both in mice and rat experiment that myosin can induce the autoimmune inflammatory reaction. A six months follow up of the patient with positive anti-MHC antibody by pang[1] found that; In the anti-MHC antibody positive group the mortality,rate of vntricular aneurysm formation,extenuate extent of ventriclar motion is significantly higher than the negative group. those hint autoimmune is involved in the cardial impairment and remodel process. chemokine CXCL16 is a CXC family chemokine was being found in the plaque of atherosclerosis recent years it can not only exist in the form of membrane-bound protein, but also can be secreted to the extracellular fluid in the form of soluable protein, it function as the scanveger receptor when in the form of membrane bounding protein, bounding and internalization low density lipoprotein and bacterium; It function as chemical inductor of activated CD4+ CD8+ T lymph cell when in the soluable form, attract T lymph T cell, CXCR6 is its exclusive receptor, Wuttge DM[43] with the RT-PCR and histochemistry technique find out the elevated expression of chemokine CXCL16 and its exclusive receptor CXCR6 in the lesion of human and apoE deficient mice atherosclerosis plaque. The experiment in vivo and in vitro has verified the expression of CXCL16 can be induced by the inflammatory factor IFN-γ. it suggest that CXCL16 and CXCR6 may play an important role in the link of inflammatory and immune reaction .Objection: to study the expression of chemokine CXCL16 and its specified receptor CXCR6 after the acute myocardial infarction, revelate the expessin the these chemokine with relation to the time.Methods: The experiment was form with two part: 1,Human myocardial specimen experiment; 2,Rat animal experiment. part one: paraffin wax spicemen provided by the pathology department of basic medicine JILIN university, 9 with myocardial infarction come from 9 individual which was further testified in the HE stain, set 6 case normal myocardial as control. use the histochemistry method to study the expressin of CXCL16 and CXCR6 in the human myocardial infarction tissue in the protein leval. part two. Rat animal experiment: 49 wistar rat randomly divided into sham,1day,3days,1week,2weeks,4weeks and 6weeks group 7 rat in each group, We establish the model of rat AMI with the ligation of LAD, To measure the expression of the CXCL16 and CXCR6 mRNA in the marginal zone of AMI areas with the RT-PCR method.Result: the histochemistry of human AMI tissue demonstrate the expression of CXCL16 and its exclusive receptor CXCR6 was significantly higher than non AMI group, The animal experiment illustrate the mRNA expression of CXCL16 was significantly higher than the sham group in the 3days,2weeks,4weeks group; The mRNA expression of CXCR6 was significantly higher than the sham group in the 3days,2weeks,4weeks and 6weeks group, The expression in 3day and 2week group reach the peaks. Conclusion: CXCL16 and CXCR6 was involved in the inflammation reaction, and play an important role. the fall and rise in the 4weeks may suggest that CXCL16 may mediate the autoimmune reaction after AMI.