Studies On The Volatile Oil Of Atractylodes Lance

This thesis utilized genuine predominance to study extraction techonology, chemical composition and assaying in Atractylodes lancea(Thunb)DC. with modern scientific means ; compared Atractylodes lancea(Thunb)DC. in different places , grow wild and cultivating samples as well as different years samples with natural resources of Chinese medicinal materials technique to estimate quality; established Atractylodes lancea(Thunb)DC. quality evaluation with finger print to guarantee Atractylodes lancea(Thunb)DC. quality stabilization and clinical medication safe , and provided ground investigation information,and provided underlaying research information . Main research contents and conditions as follows:1,SFE-CO₂ exteacting the volatile oil as index,the best extraction chrology with simple factor and orthogonal experiment:extraction pressure is 30MPa,extraction temperature is 45℃,the CO₂ flow-rate is 3L·min,extaction time is 3．5h,in this conclusion ,the quality and yield of volatil oil were best.The yield of oil ,the,yiel of Atractylon,Hinesol,β-Eudesmol,Atractydin and GC-MS relative percent content as index,we compared SD,SX,SC,SFE-CO₂ four extract methods.The result showed that the yield of oil ,the components and main components all were best with SFE-CO,which were better than other methods.2,we established the prepared method of Atractylon,Hinesol,β-Eudesmol,Atractydin of different places .The result showed that the content of Atractydin was highest in Maoshan to achieve 31．48g/g ,which reflected the quality was best in genuine place.3,We established GC-MS analytical method,and analysised genuine Atractylodes lancea(Jhunb)DC.,different growth years,grow wild and cultivating Atractylodes lancea(Thunb)DC. The results showed that chemical coponents and main components in different places were different.The content of Atractylodin and chemical compunds in genuine place were best,the main compounds match was (0．89～1．12) : (0．11～0．15) : (0．48～0．61) : 1 in Maosha.Along with time going,the main compunds will increase,but the velocity of increase were different .The main copmpounds and content of Atractylodin in grow wild samples were more than cultivation samples,the content of Atractylon in cultivation samples were more than grow wild samples , Hinesol andβ-Eudesmol were fairyly.4,We established genuine Atractylodes lancea(Thunb)DC. standard finger print with the technology of Chinese crude drug finger print and cluster analysis method ,compared the finger prints in different places and studied cluster analysis.The result showed that :The finger print of genuine Atractylodes lancea(Thunb)DC. was evident markable.The finger print can be devided into four districts,every district had its own feature,and the main peaks area were very markable, Atractylodes lancea(Thunb)DC.in different places,the finger prints of Atractylodes lancea(Thunb) DC. were different,but they were near.Atractylodes lancea(Thunb) DC.of other places compared to genuine Atractylodes lancea(Thunb) DC., the sequence of semblances of different places as follows:Tangshan,Nanshan,Yingshan,Luotian,Bozhou. SPSS cluster analysis showed that Maoshan Atractylodes lancea(Thunb)DC.,Tangshan,Nanshan cluster were main category,Bozhou,Luotian and Yingshan were main category.