Exploration Of The Relationship Between Intestinal Flora And Hyperlipidemia Through The Changes Of Large Intestinal And Small Intestinal Flora Of Hyperlipidemia Rats Model

Objective:To observe the changes of both large intestinal and smallintestinal flora of hyperlipidemia SD rats, compare the elevation of serumlipid of antibiotics intervention group and bacteria strain interventiongroup in order to approach the latent capability in accommodating serumlipid of intestinal flora.Method:50 SD rats were randomly divided into 5 groups. Group Awere normally fed,while Group B,C,D,E were fed by forage rich in oiland fat. Group B received antibiotics intervention by arilin andnorfloxacin intragastric administration from the 1st day on. Group D andE had been receiving intragastric administration of bacteriaⅠandⅡrespectively since the 15th day. We analyse the fecal microbial populationat the 1st day, the 15th day, and the 30th day, survey the serum lipid at the 1stand the 30th day. On the 30th day, we executed the rats and analyse thesmall intestinal flora.Results:The level of TC,LDL was elevated significantly inGroupB,C,E compared to its primary concentration,the increment ofTC(ΔTC) in these groups was higher than GroupA (P＜0.05) .InGroupD,the elevation of TC,TG was not significant as well asΔTC(P＞0.05).The increase of bodyweight in GroupB,C,E was significantlyhigher than GroupA, while GroupD was not apparently higher thanGroupA (P＞0.05) .The fecal microbial population changed a lot in GroupB,C,D,E,the counts of Lactobacillus acidophilus,Bifidobacteria,Enterococci was significantly decreased,the counts of Bacteroides wasalso decreased after a increasing period (P＜0.05) .The small intestinalflora was relatively stable,with just Bacteroides decreased in GroupC. Butin groupD,the counts of Bacteroides,Enterococci,Escherichia coli andStaphylococcus aureus was significantly decreased compared to GroupA.Conclusion:1.Feeding the SD rats with mixed forage made of 1% cholesterol,5% animal oil,10%egg yolk powder,0.2% Sodium Cholate and 84% basicforages for 30 days can establish hyperlipidemia rats model.2.High lipid diet intake for 30 days can result in the changes ofintestinal flora which mainly occurred in large intestine and wasconsidered to possibly accelerate the development of hyperlipidemia.3.Bacteria strain I may have the latent ability to affect lipid and fatmetabolism in small intestine,and was considered to be a candidateprobiotic.