Effect Of Balance Training On Cerebral Cortex And Balance Function Of Mouse

Balance is a basic hunman exercise skill .Balance function is especially important to retain all kinds of gestures and action in a daily life, and to proper reaction to external interference.The impairment of balance function affects allomeric function of human and even impossibly leads to fall down. Balance function is an important function of human.All kinds of action in a daily life ,such as standing and walking,depend on effective balance. It is governed by central nervous system(CNS) and adjusted by various reflex activity,peripheral proprioceptive sense and optesthesia,besides of coordinating construction among muscle groups.Thus ,balance is a set of complex course.To keep normal balance,brain needs introduce sensory information from visual system,vestibular system and proprioceptive sense of body,and brain accordingly sends out singal to propotional muscle group which keeps on balance of bearing surface.With increasing of age,balance fuction gradually decreases and especially from middle-age to old-age there are many phenomena including unsteadiness of standing and walking,decreasing scope of joint movement and weakness of absolute muscle ability and ligament elasticcity.These phenomenona are main reasons for falling and falling is an important reason for damage and other disease ,which bring extreme pain to people, and big burden to famliy and society.In a word,balance function is important in human's llife. The purpose of this study is to observe the effect of balance training on cerebral cortex and balance function of mouse.Methods Sixty C57BL/6J female mice were randomly divided into the control group,A group ,B group and C group with 15 animals in each group. A group was trained by revolving balance training which respectively turned to left and right .Velocity was 5 rolls per minute.These mice were trained 10 mintues in the frist day and everyday incresed 10 mintues untill to 30 mintues.6 days per week and total time was 2 weeks.Method of B group was the same with the of A group and total time was 4 weeks.C group was trained by revolving balance training which respectively turned to left and right . Velocity was 5 rolls per minute,too. These mice were trained 10 mintues in the frist day and incresed 10 mintues everyday untill to 60 mintues. 6 days per week and total time was 4 weeks. Raising conditon of the four groups was all routine raising but the control group was not trained by revolving balance training. The balance function,brain index, pathology observation and the content of MDA and activity of superoxide dismutase(SOD) in the mouse cerebral cortex were tested after 2 weeks and 4 weeks training.Results Ethology detection: The total time passing balance beam of B group and C group significantly shortened compared with the control group(P<0.01).Compared with A group ,the total time passing balance beam of B group and C group both shortened ,especially B group.Experimental result of water maze indicated that there was no significant difference about all time of swimming and frequency of error in the control and balance training groups.This balance training couldn't effect the spatial cognition and the learning achievement.Cerebral index: Compared with the control group,cerebral index of A group increased(P<0.05),and B group and C group also increased significantly (P<0.01).The change of cerebral morphology in mice indicated that nerve cells of cerebral cortex in the groups stained uniformly.Their construction was integrity.Cellular membrane and caryotheca of nerve cells was clear.Compared with the control group, nerve cells of cerebral cortex in the training groups arranged more closely,cell nucleus was rounder and bigger,caryoteca was clear and tigroid body in intracytoplasm was abundant . Compared with the control group, the SOD activity of cerebral cortex significantly increased(P<0.01),and MDA decreased(P<0.05) in the B group.Compared with the control group, the content of MDA in the C group decreased(P<0.05). Compared with the A group, the SOD activity of cerebral cortex increased(P<0.05) and the content of MDA decreased (P<0.05)in the B group.Conclusions These results suggested that the prolonged balance training could improve balance function ,increase the brain index and decrease lipid peroxidation level in the cerebral cortex of the mice. The moderate amount of prolonged balance training initiated could significantly improve cerebral function and protect nerve cell ,which was a kind of aerobic training.