Expression And Signification Of Anti-apoptosis Proteins Survivin And Ki-67 In Adenomyosis

Objective To investigate the expression of Survivin protein and ki-67 in eutopic and ectopic endometria and smooth muscle tissue in adenomyosis in order to study the function and relative signification of apoptosis,cell proliferation in the course of adenomyosis.Methods Patients were random selected from the department of gynaecology and obstetrics in the first affiliated hospital of dalian medical university from October in 2004 to October in 2006.Tisstes samples included 30 samples of ectopic endometriums (17 cases in the proliferative phase,13cases in the secretory phase)and eutopoic endometriums(13cases in the proliferative phase,17cases in the secretory phase) of patients with adenomyosis, and 18 samples of normal endometrium tissues(11cases in the proliferative phase,7cases in the secretory phase). Using streptavidin-biotin peroxidase(S-P)method of the immunohistochemistry, we examined the expression of the Survivin proteins and Ki-67 in these tissues. Statistical analysis was performed by using SPSS11.5 statistics software.Results1.The expression of Survivin proteinThere was expression of Survivin protein all together in ectopic and eutopic endometrium of adenomyosis and control's normal endometrium which was most observed in cytoplasm of glandular epithelial cell. The expression of Survivin protein in glandular epithelial cell on ectopic endometrium of adenomyosis was significantly higher than that of eutopic endometrial and normal endometrium (P=0.017,P<0.05) , there was significant variation between the latter two groups(P=0.000,P<0.05),and these variation was mainly showed in the proliferative phase;There was nocyclic change in eutopic and ectopic endometrium of adenomyosis(P>0.05); There was small amounts expression and no cyclic change(P=0.057,P>0.05) in glandular epithelial cell on concrol's normal endometrium.The expression of Survivin protein in stromal cell of three groups above had no significant diffrence(P=0.507,0.698,0.818,P>0.05) and no cyclic change(P=0.444,0.733,0.771,P>0.05).There was small amounts expression in smooth muscle cell of adenomyosis and control . There was no significant variation (P =0.331,0,545,P>0.05)and no cyclic change as well(P>0.05) between them.2. The expression of Ki-67There was expression of Ki-67all together in three groups above which was most observed in cytoplasm of glandular epithelial cell. The expression of Ki-67in glandular epithelial cell on ectopic endometrium of adenomyosis was significantly higher than that of eutopic endometrial and normal endometrium (P = 0.006,0.033,P<0.05), but there was no significant variation between the latter two groups(P=0.622,P>0.05); There was no cyclic change in eutopic and ectopic endometrium of adenomyosis(P>0.05), whele the expression of Ki-67 has marked vary during the menstrual cycle in concrol's normal endometrium ,which is higher in the proliferative phase than in the secretory phase) (P=0.017,P<0.05).The expression of Ki-67in stromal cell of three group above has no significant diffrence(P= 0.507, 0.698, 0.818, P>0.05) and no cyclic change (P>0.05).There was small amounts expression in smooth muscle cell of adenom- yosis and control , There was no significant variation (P = 0.316, P>0.05)and no cyclic change(P>0.05) between them.3 .There was a poor correlation between survivin and Ki-67 expression levels in 30 glandular epithelial cells on ectopic endometrium of adenom- yosis examined(r=0.311,P=0.015,P<0.05) .Conclusion1. The overexpression of Survivin protein and Ki-67 may take part in the formation of adenomyosis and Survivin-collected apoptosis and Ki-67-collected cell proliferation might play a pathogenic role in the development of adenomyosis.2 The eutopic endometrium of adenomyosis in the secretory phase may be of more proliferative ability and may play important role in the format- ion of ectopic endometrium .3 The association of the expression of Survivin protein and Ki-67 in glandular epithelial cell on eutopic and ectopic endometrium of adenomy- osis may be of occurrence in one of adenomyosis pathogenesis.