Investigation On The Neuroprotective Effects Of The Chemical Constituents From Spiraea Japonica Var. Acuta In Vitro

Objective: To search for the neuroprotective constituents from Spiraea japonica var. acuta and investigate their mechanisms of neuroprotection . Methods: Four fractions (A, B, C, and D) and four compounds (spiramine P, Q, T, and U) from 5. japonica, were tested for neuroprotective activities against OGD-induced impairment in PC₁₂ cells. The protective mechanisms of anti-excitotoxicity and antioxidant were studied by using L-glutamic acid and peroxide hydrogen (H₂O₂) induced impairment in PC₁₂ cells. Furthermore, the LDH(lactate dehydrogenase) leakage and the activity of SOD (superoxide dismutase )in H₂O₂-induced injury in PC₁₂ cells were also evaluated. In addition, whether the constituents directly react with H₂O₂ or not was examined by the convenient test of H₂O₂.Results: Preincubation of PC₁₂ cells with the fractions of C and D significantly protected against the cytoxicity induced by OGD. The C also prevented cytotoxicity induced by H₂O₂. In addition, treatment with C prevented the release of LDH and increased the activities of SOD in H₂O₂-injured PC₁₂ cells, and the C didn't react with H₂O₂ in the cell-free system.Conclusions:The C and D had the neuroprotective effect in vitro. The neuroprotective mechanism of the C was prevented cytotoxicity induced by freeradicals.