| Rheumatoid arthritis (RA) is the current world within the scope of highly damaging one of the chronic disease, is a kind of refractory autoimmune diseases, etiology is unclear.Objective Treatment of the disease has the advantages of traditional Chinese medicine, leaves false gentian in clinical has better anti-inflammatory effects, radix gentianae macrophyllae is (to medicine. This topic is based on false gentian pointed leaves with radix gentianae macrophyllae compatibility as the research object, with RA as the breakthrough point, on the false gentian pointed leaves with the compatibility of radix gentianae macrophyllae anti-inflammatory immune function and the mechanism of the treatment of RA has carried on the thorough research.Methord Experimental method:this topic research object for the SD rats induced by the CIA, to more positive selection dr ug tripterygium wilfordii glycoside, randomly divided into norm al control group (group C), model group (M), tripterygium wilfo rdii glycoside groups more group (TG), leaves fake gentian com patibility gentiana macrophylla (1:1,1:2,2:1). In 1 day,7 days in the rat tail root injection type C II collagen/IFA building, starting from 14 days to fill the stomach 18 days. Measurement s at each time point in the rat body weight, volume measureme nt of rats after the right toe swelling volume, spleen pathologic al HE, the rat serum IFN-gamma Elsia method and TNF alpha, IL-4, IL-10 levels, immunohistochemical method synovial ti ssue ICAM-1, VEGF, MMP-3 protein expression.Consequence1, Gentianella acuta and Gentiana compatibility can increase weight of CIA rats and reduce the CIA rat paw swelling, reduce the CIA rat arthritis score and reduce the generation of CIA rat spleen white pulp.2. Gentianella acuta and Gentiana compatibility can be by reducing the content of CIA rats serum IFN gamma, THF alpha and enhance the content of serum IL-4 and IL-10 regulate Th 1/Th2 balance, the 2:1 group effect is the most obvious. (P<0.05)3, Arthritis scoring:each group compared with group C, the CIA arthritis scores were significantly increased (p< 0.01).3. Gentianella acuta and Gentiana compatibility can decreas e the expression of CIA rat synovial tissue ICAM-1, Vega, MM P-3 protein, thereby inhibiting the inflammation and proliferatio n of synovial membrane and reduce the formation of pannus, pr event cartilage erosion. And promoting the Fas/FasLmRNA expre ssion in the synovial tissue of CIA rats to improve the CIA rat ’s synovial cell apoptosis. The most obvious effect in 2:1 group (P<0.05 P<0.01).4. Gentianella acuta and Gentiana compatibility can lower serum DKK1 levels in CIA rats, thus affecting the Wnt pathway to reduce bone destruction.1:2 group significantly reduced (P< 0.05, P< 0.01).Conclusion:The compatibility of gentianella acuta and Gen tiana has multiple levels, anti-inflammatory and immunomodulati ng effects of multi way and multi targets. Its anti-inflammatory, immune regulation and its inhibition of inflammatory factors to regulate the proportion of TH1/TH2. The expression of synovia 1 tissue ICAM-1, Vega, MMP-3 protein regulation and of apopto sis factor Fas/FasLmRNA expression may is the control synovial inflammation and pathological basis to prevent the pannus form ation and cartilage destruction. And the regulation of Wnt signa ling pathway to alleviate joint inflammation and bone destructio n. |
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