Experimental Study On Reticulocyte Predicting Bone Marrow Function

OBJECTIVE: By experimental study on rats, explore the relationship of changing time between various kinds of RET values and other blood cells values on the phases of bone marrow inhibition and recovery. So a more sensitive indicator of forecasting marrow function is found to guide doctors using G-SF or GM-SF timely when treating tumor by chemicals.METHODS: 1. Walker cancer No.256 cells are vaccinated to subcutis of 36 rats' right armpit to raise tumor models.2.By means of abdominal cavity perfusion, all rats are treated with Cyclophosphamide(60mg/kg) single time.3.After chemotherapy, WBC, ANC, PLT, RET#, RET%, LFR,MFR and HFR are observed dynamicly by SE-9000/RAM-1 till marrow function of all rats recovering to normal.RESULTS: After chemotherapy, marrow function of rats are inhibited obviously . Time of HFR descenting statisticly(P<0.05,)is 0.5 day earlier than WBC's and ANC's and is 1.5 days earlier than PLT's. Time of RET#,RET%,MFR and LFR changing statisticly (P<0.05)is 0.25 day earlier than WBC's and ANC's and is 1.25 days earlier than PLT's. On the phase of marrow function recovery, time of RET#, RET%, LFR, MFR and HFR changing statisticly(P<0.05,)is 1 day earlier than WBC's and ANC's and is 2 days earlier than PLT's.CONCLUSION: RET, especially HFR, is the sensitive indicator to reflect the beginning of marrow function inhibition and recovery, which can be used to guide doctors using G-SF or GM-SF timely when treating tumor by chemicals.