Studies On Perilla Frutescens(L.) Var. Frutescens's Function Of Hepatic Protection And The Initial Isolation Of Active Components

Perilla frutescens(L.) var. frutescens belongs to the genus of Perilla Linn.o Used as an alternative of Perilla in Sichuan and Guizhou Provinces, Perilla rutescens(L.) var. frutescens is a traditional Chinese herbal medicine with a long history of application. It abounds in effective components and has a great potential value of development.lt is known that stewed seeds of Perilla frutescens(L.) var. frutescens with duck meat is widely used among civilians as a anagraph for curing hepatopathy and hepatocarcinoma. to find a development direction for Perilla frutescens(L.) var. frutescens and improve its economic value, we studied the function of hepatic protection and conducted an initial isolation.To study the hepatic protection, we established a carbon tetrachloride-induced acute hepatic injury animal model of Kunming mouse. Comparing the changes in the weitht of all groups of mice, we found that the weight of all groups of mice showed an obvious decrease within 24 hours after model establishment. Until day 5, the comparison between weithts of the model group, the low dosage group and the showed no apparent difference. The weight of the medicine-positive group and the high dosage group restored at day 3, with no apparent difference. The ALT and AST activity in blood serum showed an obvious increase after model establishment; The ALT and AST of model group increased by 23 and 14 folds, respectively, compared with the solvent. The Deritis ratio decreased significantly, from 1.667 in the solvent control group to 1.027 in the model group. Theexperiment mouse given medicine for a successive duration of a week, the activity of ALT and AST reduced in the medicine-posibtive group, with an apparent reduction in the high and medium dosage groups and the medicine-positive group compared with the model group(P<0.05), where the reduction of ALT activity was 53.06%, 20.64% and 49.7%, respectively, and the reduction of AST activity 46.07%, 45.2% and 51.2%, respectively.The result demonstrated that Perilla frutescens(L.) var. frutescens really functions in reducing ALT and AST. We conducted physiological examination on hepatic tissue section , making assessment in the degree of hepatic injury of each tissue sections, according to the standards of judging hepatic injury.(P<0.01)We made a relative analysis of medical efficiency in the model group, the medicine group, and medicine-positive group, checking statistically using Ruidit value, and found an extreme difference (P<0.01). According to the above findings we conclude that the effective components of Perilla frutescens(L.) var. frutescens plays an apparent and reliable part of curing the carbon tetrachloride-induced acute hepatic injury.a-amanitin intoxication can increase the viscera index of visceras such as liver during the intoxication anaphase. Here, we also examined the pharmacological effects of the Perilla frutescens(L.) var. frutescens seeds extract by acetic acid on the a-amanitin induced hepatic injury. Four immune and apoptosis related genes CatnB, Flt3L and Rpo2-4 were selected as research objects to observe their transcriptional variations before and after using test medicine. Studies indicate that the medicine down-regulated CatnB and up-regulated Flt3L to a certain degree. Studies also show that the test medicine did not have an apparent effect on the expression of Rpo2-4.Studies on Perilla frutescens(L.) var. frutescens's function of hepatic protection and the initial isolation of active components explained scientifically the hepatic protection of Perilla frutescens(L.) var. frutescens from pharmalogical and physiological perspectives and made conclusions for the first time It also confirms the notion in documents and taxology that Perilla frutescens(L.) and Perilla Lin are not identical plants and should not be used as the same medicine. The study lays asolid foundation for the development and application of Perilla frutescens(L) van frutescens.