Study Of Chemical And Anti-cancer On The Perilla Frutescens(L.)Britt.

Perilla frutescens(L.)Britt., is a member of Perilla genus which belongs to the family of Lamiaceae, annual herbaceous, distributed widely in our country, except of Hainan and Xinjiang. The resource is rich, output is large. The seeds, stems and leaves can be used as medicine, and they are recorded by Chinese pharmacopoeia and can cure many diseases. To select the plant resources which riched in α-linolenic acid, using the content of α-lino- lenic acid as an indicator, the consent of fatty acids in 23 kinds of plant oils were analyzed by GC, the results find that perilla seed oil is the highest in the content of α-linolenic acid, reaching 64.42%; The preliminary researches of our group find that the alcohol extract of stir-fried perilla seed can enhance the ant oxidative ability and find that the anti free radical active components,namely multiple phenolic components; For reasonable using Perilla seed resources,this article uses the single factor investigation(temperature and time), using Perilla alcohol extract of total polyphenol content as an index, the optimal process conditions are as follows: temperature 180℃, time 2 min, the Perilla seed reached the highest content of total polyphenol that is 0.54%;In order to study the effect of α-linolenic acid-induced apoptosis in the human leukemia U937 cells and the molecular mechanism of action, the human leukemia U937 cells were treated with different concentrations of α-linolenic acid in the experiments. After the human leukemia U937 cells were treated for 24 hours, the cell proliferation was measured by MTT assay. We found that the cell proliferation of human leukemia U937 cells was significantly inhibited by α-linolenic acid, and the correlation between inhibitory effect and the concentration of α-linolenic acid dose was positive. And we detected the effects of α-linolenic acid inhibiting cell proliferation and inducing apoptosis by DNA fragmentation analysis and flow cytometry, as well as we detected that whether α-linolenic acid could activated the down regulation of MMP, the caspase’s activity and ROS generation. And the proteinexpression of apoptosis induced by α-linolenic acid also was detected. We found theα-linolenic acid could induce the down regulation of MMP, the caspase’s activity and ROS generation. The expression of Bax protein was increased byα-linolenic acid.And the expressions of Bcl-2 and Bid protein were increased byα-linolenic acid. In conclusion, the α-linolenic acid-induced apoptosis in U937 cells was related to the caspase-dependent mitochondrial pathway.