Effects Of Environmental Estrogen Diethylstilbestrol On The Expression Of WISP-2 Gene And Role Of WISP-2 Gene On The Expression Of BCL-2 Gene In Human Breast Cell Line MCF-7

Object:l)To explore the effects of diethylstilbestrol on the expression of the WISP-2 gene in human breast cancer cell line MCF-7,and to compare its effect with 17- β estradiol. The research is to study the role of diethylstilbestrol in the development of human breast cancer, and its mechanism.2)To explore the effects of WISP-2 gene on the expression of the BCL-2 gene in human breast cancer cell line MCF-7. The research is to study the role of them in the inhibition of cell apoptosis and acceleration of cell proliferation .Methods:MCF-7 cell were cultured in vitro, and exposed to various concentrations of diethylstilbestrol in the different time, the expression of WISP-2 mRNA and its protein were measured using RT-PCR and immunohistochemistry approach. The experiment set up vacuity negative control , and 17-β estradiol positive control, the expression of BCL-2 protein was measured using immunohistochemistry approach when the expression of WISP-2 protein changed in the MCF-7 cell. To detect the proliferation was using MTT approach.Results:1) Diethylstilbestrol accelerated the MCF-7 cell proliferation.2) Exposed to diethylstilbestrol, expression of WISP-2 mRNA and its protein was increased. The effect of high concentration diethylstilbestrol was...