The Expression And Significance Ofβ-catenin、Wisp-1 And Caspase9 In Human Colon Cancer Tissue

Objective：To explore the gene，protein expression ofβ-catenin、wisp-1、caspase-9 in Colon tumor and analyze the relationship between theexpression and clinical pathology of Colon tumor，correlations between theβ-catenin、wisp-1、caspase-9 protein and gene.Methods：The protein expression ofβ-catenin、wisp-1、caspase-9 weredetected by immunohistochemistry（IHC）in 138 cases of primary coloncancer tissue、37 cases of colon adenoma tissue and 66 cases of normal colonmucosa tissue；the mRNA expression ofβ-catenin、wisp-1、caspase-9 weredetected by real-time fluorescence quantitative PCR（RT-PCR）in 37 cases offresh primary colon tumor and 37 cases of fresh normal colonic mucosa；Theexperimental results combined were statistically analyzed with the clinicalpathological sample data.Results：1. All the statistics of the positive expressions ofβ-cateninprotein in colon cancer、colon adenoma and the tissue of adjacent coloncancer were higher than that in normal colon mucosa (p<0.05)；The positiveexpressions of wisp-1 protein in both the colon cancer and the tissue ofadjacent colon cancer were higher than normal colon mucosaexpression(p<0.05); The strong positive(≥++)of bothβ-catenin and wisp-1protein in Colon cancer was higher than that in colon adenoma and the tissueof adjacent colon cancer (p<0.05). The positive expression rate of caspase9 protein in colon cancer，colon adenoma，tissue of adjacent colon cancer andnomal colon tissue shows a rise trend，but comparing the expression rate ofeach group，there is no statistically significant difference (p>0.05)；comparingthe strong expression rate(≥++) of each group no statistically significantdifference either (p>0.05). 2.β-catenin protein expression had closerelationship with clinical stages、infiltration depth and lymph nodemetastasis(P<0.05)，there is no significant correlation betweenβ-cateninprotein positive expression and sample’s gender，age，tumor types anddifferentiation degree (p>0.05).wisp-1 protein expression just had closerelationship with infiltration depth and lymph node metastasis(P<0.05)，butno significant correlation between wisp-1 positive expression rate andsamples’gender，age，tumor types，differentiation degree and tumor location(p>0.05)；Caspase9 protein positive expression rate has no significantcorrelation with each clinical index. 3.In colon cancer，although the averageexpression ofβ-catenin、wisp-1、caspase9 mRNA were respectively1.946，1.349 and 1.587 times the level in matched normal colon tissue，butonly the result ofβ-catenin mRNA difference had Statistics Meaning(P<0.05).β-catenin mRNA expression had close relationship with tumorlocation、tumor types and lymph node metastasis(P<0.05)，yet had norelationship with gender、age、infiltration depth and differentiation degree(p>0.05).wisp-1 and caspase9 mRNA expression only had relationship withtumor types and no significate relationship with any other of pathologicalfeatures.4.There were positive correlations betweenβ-catenin protein andwisp-1 protein（r=0.423，p < 0.001），β-catenin mRNA and wisp-1 mRNA (r=0.461，p<0.005)，Wisp-1 protein and caspase9 protein（r =0.183，p<0.05），Wisp-1 mRNA and caspase9 mRNA（r =0.526，p<0.001），respectively. There were no correlations betweenβ-catenin protein andcaspase9 protein（r =0.110，p > 0.05），β-catenin mRNA and caspase9mRNA（r =0.094，p > 0.05）.Conclusions：1. Both ofβ-catenin protein and mRNA show highexpressions，which with their clinical features suggests thatβ-catenin may becorrelated with the development and diagnosis of colon tumor. 2. The wisp-1protein in colon tumor shows high expression which is obviously higher thanthat of Colonic adenoma and normal mucosa tissue and closely correlated totumor infiltration depth，lymph node metastasis. This suggests that thewisp-1 protein may be correlated with the development and diagnosis ofcolon tumor. However，there is no significant difference between theexpressions of wisp-1 mRNA in colon cancer and the normal colonic tissue，which indicates that the role of wisp-1 mRNA is not clear in the developmentof colon tumor.3.This is no significant difference in protein and mRNAexpression levels of caspase9 in colon cancer，colon adenoma and normalcolonic mucosa，which suggests the role of caspase-9 in the development ofcolon cancer was not clear either.4. The protein and mRNA expressions ofβ-catenin and wisp-1 have a positive correlation，which suggests thatβ-catenin and wisp-1 are involved in the development of colon cancer，eitherjointly or independently.