Treatment Of Rhodiold Sachalinensis On Glomerular Nephritis

The disequilibrium of the process of the glomerular extracellular maxtrix (ECM) synthesise and degradation is an important mechanism which cause the gatheration of the glomerular ECM and lead to glomerulosclerosis. Plasminogen activator (PA) and fibrinolysin system belongs to the serine stretch protein enzyme system and it is one of the most important degradation systems of ECM. Plasminogen activator inhibitor type-1 (PAI-1) is PA's specific physiological inhibitor and plays an important role in the occurrence of glomerulosclerosis. Tripterygium wilfordii hook as the genus plant of Tripterium Regelii has a wide clinical use, but Tripterium Regelii is used little clinically for its heavy toxicity. We haven't seen the article about the therapeutical effect on glomerulonephritis with Tripterium Regelii. The prupose of this study is to observe the change of levels of urine protein, liver function, renal function, cholesterin, blood white cells in nephritis models of kidneys and the influence of PAI-1 in the renal tissue homogenate on the glomerulonephritis, to make sure the characteristics of Tripterium Regelii, to provide theoretical evidences for developing Tripterium Regelii further.Divide 30 rats into 7 groups at random: (1) normal control group(n=10); (2) model control group(n=10): after inducing nephritis model's rats successfully with small doses of adriacin many times. We treated them with no drugs; (3) model treatment group(n=10): After successfully inducing nephritis in rats with small doses of adriacin many times, we treated them with the solution of Tripterium Regelii 1.5ml per day(equal to the primary Tripterium Regelii20 gram per kg body weight) for 28 days, collect 24 hours' urine and blood sample, take out kidneys of all rats. (4) poison experiment group B(n=10):give Tripterium Regelii solution 0.75ml per day (equal to the primary Tripterium Regelii 11.25gram per kg body weight); Collect 24 hours' urine of all rats with metabolism cages before and after the experiment determine the content of urine protein with the method of sulfosalicylic acid turbidimtry, We employed color developing substrate to determine levels of PAI-1 in renal tissue homogenate.