The Experimental Study Of Infantile Rats With Ulcerative Colitis Of Spleen Deficiency Type Treated By Jianpihuashu Decoction

ObjectiveTo establish rat ulcerative colitis of spleen deficiency model and observe abnormal NO, NOS, SOD, MDA in colon constitutionMethods60 SD rats were divided into 5 groups: normal control (group A), experiment (ulcerative colitis (group B) and ulcerative colitis of spleen deficiency type (group C)), treatment (JPHS (group D) and SASP (group E)), sienna leaf oral liquid was administered in group C, D, E to establish model of spleen deficiency. The group B, C, D, E were respectively received saline enemas, JPHS, SASP enemas 1 ml daily after distal colitis was included by 5% acetic enemas was study by pathology and biochemistry, respectively.Results1 In rats of group B, C, obvious hyperemia, edema in colon mucous and sub mucous were found, and obvious infiltration cells, decrease of glandular goblet cells and formation of typical ulcer could be seen while in grounded, there were few ulcers, and the remaining ulcers presented obvious recovering changes, such as recovery of mucous, hyperplasic of granulation and cicatrisation. The damage score in experiment group was notably higher than that in treatment group (P<0.01). There were also significant difference between treatment group and control group (P<0.01).2 The levels of No and activities of NOS in colon mucous of the rats in experiment group were significantly higher than those in control group and treatment group (PO.01), But there was no significant difference between control group and treatment group (P>0.05)3 MDA in experiment group was significantly increased than that in control group and treatment group (P<0.01), but SOD in experiment group was significantly lower than that in control group and treatment group (P<0.01). There was no significant difference between control group and treatment group (P>0.05)ConclusionJianpihuashi decoction has better effects in treatment of ulcerative colitis in consideration of the aspects of clinical overall effect, manifestation, intestinoscopy mucous change and mucous pathological histological research. To reduce free radical which involved in the pathological process of the disease can provide a new pathway for ulcerative colitis.