Study On Effect Of Processing Technology On Probiotics And Application Effect Of Probiotics

The experiment was conducted to study the effect of different drying ways and different condition time in pelletion technics on live-microbe amount in probitics.The probitics were fed to the growing pig to determine the practical effect of it.1 Effect of processing technology on probioticsThe effects of different drying ways including air-dry,drying and freeze-drying on live-microbe amount in probitics showed as follows:The group dealt with freeze-drying method in all treatment has the largest number of live-microbe amount in probitics.The logarithm of Lactobacillus(lg^CFU/g) is 8.00 in probitics dealt with freeze-drying method,Yeast(lg^CFU/g) is 7.00 and Bacillus(lg^CFU/g) is 8.14.The least number of live-microbe amount in probitics is the group dealt with drying method under 65℃in all treatment.The logarithm of Lactobacillus(lg^CFU/g) is 5.21 in probitics dealt with drying method under 65℃,Yeast(lg^CFU/g) is 5.09 and Bacillus (lg^CFU/g) is 8.09.The number of Lactobacillus and Yeast in probitics had decreased very significantly with the methods of air-dry and drying compared to the method of freeze-drying(P＜0.01),but the number of Bacillus hadn't(P＞0.05)The results of different condition times of 15s,30s,45s and 60s for live-microbe amount in the diets of growing pigs which supplemented with 1000mg/kg probitics during the pelletion technics process indicated that:In the absence of any protection measures,the number of live-microbe in probitics had reduced very significantly with the increase of condition time(P＜0.01).The logarithm of Lactobacillus(lg^CFU/g) is 5.89 in probitics under 15s condition time,Yeast(lg^CFU/g) is 5.93 and Bacillus(lg^CFU/g) is 6.65.The logarithm of Lactobacillus is 4.24 in probitics under 60s condition time, Yeast(lg^CFU/g) is 4.22 and Bacillus(lg^CFU/g) is 5.13.2 Effect of probiotics on performance and microbes in dejectas for growing pigs150 growing pigs(Large white×Duroc×Landrace) were divided into 5 groups (a blank group,a control group and three trial groups) with three replicates per treatment and ten pigs per replicates.The blank group was fed with corn-soybean meal;the control group was fed with basal diets adding antibiotic(100mg/kg kitasamycin and 300mg/kg terramycin);the experiment groupⅠwas fed with basal diets adding probitics in 500mg/kg level,the experiment groupⅡwas fed with basal diets adding probitics in 1000mg/kg level and the experiment groupⅢwas fed with basal diets adding probitics in 1500mg/kg level.The results indicated that:Compared with basic group,0.15%addition of probitics in diet of pigs can improve the growth performance and increase apparent digestibility of nutrients of growing pigs'.The feed conversion ratio reduced 5.82%(P＜0.05);the rate of diarrhea decreased 35.14%(P＜0.05 );the digestibility rate of crude protein,Calcium,Phosphorus and crude fat increased 3.82%,3.62%,2.81%,5.43%respectively(P＜0.05);the diffusion of ammona in fermented pig dejecta reduced 36.06%(P＜0.05 ).It can also improve the microbiological environment in the intestine of pigs' and promote the proliferation of Lactobacillus,Bifidobacterium and other beneficial stains(P＞0.05 ) while inhibit the proliferation of the E coli and other harmful bacteria(P＜0.05). Compared with control group,0.15%addition of probitics in diet of pigs can improve apparent digestibility of nutrients of growing pigs';the digestibility rate of crude fat increased 4.52%(P＜0.05 ),the diffusion of ammona in fermented pig dejecta reduced 25.80%(P＜0.05).Compared with treatmentⅠ,the rate of diarrhea of treatmentⅢdecreased 33.33%(P＜0.05 ),the diffusion of ammona in fermented pig dejecta reduced 31.16%(P＜0.05).The results indicated that it's feasible to replace the antibiotics with the probiotics in pigs' diets adding probitics in 0.15%level.