Study On The Combined Probiotics And Its Effects On Prodution Performance Of Pigs

Due to toxicity, residue, side effect and superbug appearance caused by antibiotics abuse, antibiotics will be moved away from feed additive. So the safe feed additives such as probiotics, Chinese herbs, antibacterial peptide and so on will become important for replacing antibiotics. Three strains of Bacillus Subtilis (B-1, B-2, and B-3) were firstly selected in this study to obtain optimal proportion. Then the combination was mixed with different proportion of Lactobacillus and yeast to make the combined probiotics, which was mixed with oligosaccharides or berberine for improving pig production. The main contents are as follows:Experiment 1 : Three strains of Bacillus Subtilis were used to study the antimicrobial effects of the different proportions on inhibiting E. coli growth. The results showed that the optimal ratio of B-1, B-2 and B-3 for inhibiting E.coli prolification was 0:1:1(P<0.05).Experiment 2: The above combination was mixed with different proportion of Lactobacillus and yeast. The inhibition of composite strains on E. coli prolification was determined by the measurment of bacterial fluid pH value and E. coli number. The results showed that the optimal ratioes of Bacillus Subtilis combination, Lactobacillus and Yeast was 0:1:1, 2:2:3 and 2:3:2 for inhibiting E.coli prolification, which were superior to antibiotics(P<0.05).Experiment 3: To study the cooperating effect of berberine and lactic acid bacteria on E.coli growth, the different concentrations of berberine were used during the period of lactic acid bacteria incubation. The results showed that 0.1% berberine in lactic acid bacteria incubation would significantly inhibit lactic acid bacteria growth (P>0.05); while 0.02% berberine in lactic acid bacteria incubation had significant effect on inhibiting E. coli prolification without affecting lactic acid bacteria growth (P>0.05).Experiment 4: To study the effects of probiotics, oligosaccharides and berberine combinations on production performance of pigs, ninety 60-day-old pigs (Duroc×Landrace×Pietrain) were assigned to 9 groups, 10 pigs for each group. The experimental period was 60 d. The basal diet was consisted of corn and soybean feedstuffs without antibiotics as the control group; and the other 8 diets were added with 0.1% aureomycin, 0.05% probiotic, 0.1% probiotic, 0.05% probiotic+0.05% oligosaccharides, 0.05% probiotic+0.1% oligosaccharides, 0.1% probiotic+0.05% oligosaccharide, 0.1% probiotic+0.1% oligosaccharides, 0.05% probiotic+0.05% oligosaccharides+0.02% berberine, respectively. The result indicated that the average daily gain (ADG) in group 2, 7 and 9 was increased by 10.61%,10.33%, 7.22% (P>0.05), and the ratios of feed intake and ADG were decreased by 5.68%, 6.43%, 6.06%, compared with the control group, indicating that the combination of probiotic and oligosaccharides had the same effect as antibiotics on improving pig growth. The diarrhea rates in group 6 and 9 were decreased by 80.5% and 66.7%, and nutrient digestibility in group 2, 4 and 8 was significantly improved (P<0.05), compared with the control group. Analysis of economic benefits indicated that the net income in group 2, 7 and 9 was incerased by 39.89, 37.73 and 26.96 Chinese yuan per pig, compared with the control.Experiment 5 : The effects of probiotics, oligosaccharides and berberine combinations on intestinal microbial flora and digestive enzyme activity were studied. The results showed that different levels of probiotics and oligosaccharides could increase the amount of lactic acid bacteria in pig feces, the addition of berberine significantly decreased the counts of E. coli in piglet feces, compared with control group (P<0.05). In addition, the combinations of probiotics, oligosaccharides and berberine significantly increased fecal lipase activity (P<0.05), but the protease and amylase activities had no significant changes (P>0.05).