Effects Of Compound Probiotics On Growth Performance And Blood Biochemical Parameters Of Growing Pigs

Environmentally friendly and non-antibiotic additives feed using in livestock-raising process are two important prospect for the development of husbandry industry.Compound probiotics,a potential antibiotic substitute,has increasingly cared by animal researchers because it originated from nature with a series of advantages including the maintenance of intestinal health,the homeostasis of bacterial flora,and the improvement of anti-oxidation and immunity.The aim of this research is to assess the effects of compound probiotics which of the main components are Enterococcus faecalis ?1×109 cfu/g,Bacillus subtilis ?1×109 cfu/g,Lactobacillus acidophilus ?1×109 cfu/g(Enterococcus faecalis: Bacillus subtilis: Lactobacillus acidophilus = 1:1:1,mass ratio)and determine the optimum addition level for growing pigs.160 “Du × Chang × Da” ternary crossbred strain pigs(weight ± SE,33.03 ± 0.28kg)with similar genetic basis were randomly divided into 4 groups:(1)control group without compound probiotics(control group,n = 10);(2)treatment with compound probiotics of 100 mg/kg in basal diet(experiment group I,n = 10);(3)treatment with compound probiotics of 200 mg/kg in basal diet(experiment group II,n = 10);(4)treatment with compound probiotics of 300 mg/kg in basal diet(experiment group III,n = 10),The experiment lasted for 28 days.Observed its growth performance and diarrhea rate,apparent digestibility,blood biochemical indicators,antioxidant,immune were measured.The results shown as follows:(1)Growth performance and diarrhea rate:The final weight and daily weight gain of pigs quadratic linear increased with the gradient additive level of compound probiotics(P<0.05).The average daily feed intake showed a linear growth trend with the increase of the compound probiotics(P<0.05).Compared with the control group,the addition of 200 mg/kg compound probiotics significantly increased the daily weight gain(P<0.05).In addition,compared with the addition of 300 mg/kg compound probiotics,the treatment group with 100 mg/kg?200 mg/kg compound probiotics that significantly decreased the diarrhea rate(P<0.01).(2)Apparent digestibility:Compared with the control group,the addition of 200 mg/kg and 300 mg/kg compound probiotics significantly increased the phosphorus digestibility(P<0.05),and the phosphorus digestibility increased linearly with the addition of the compound probiotics(P<0.01).Compared with the 300 mg/kg addition level of compound probiotics,100 mg/kg and 200 mg/kg addition level increased the energy absorption(P<0.05),and 200 mg/kg significantly increased the digestibility of crude protein(P<0.05).The total energy could increase with the increase of the amount of compound probiotics(P<0.05).(3)Effects of serum physiology and biochemical indicators:The contents of total cholesterol(TC)and total protein(TP)showed a linear trend with the increase of the amount of compound probiotics(P<0.01),and the blood glucose(GLU)content showed a linear trend(P<0.05);The activity of aspartate aminotransferase(AST)showed a linear trend with the increase of the addition amount of the compound probiotics(P<0.01).Compared with the control group,the addition of different levels of the compound probiotics extremely significantly increased the TP content(P<0.01);and 200 mg/kg compound probiotics addition level can increase the globulin(GLB)(P=0.064)? GLU content(P<0.05)and TC content(P<0.01);100 mg/kg and 200 mg/kg addition leveal of compound probiotics significantly reduced the AST compared with other groups(P<0.01).The addition of 200 mg/kg compound probiotics significantly increased alkaline phosphatase(ALP)activity(P<0.01).(4)Antioxidant:Compared with the control group,the addition amount of 200 mg/kg compound probiotics increased the content of superoxide dismutase(SOD)in serum(P=0.077).And the SOD content increased with the addition of the compound probiotics that shows a Sublinear trend(1>P>0.05).The addition of different levels of compound probiotics had no significant effect on glutathionease(GSH)content,malondialdehyde(MDA)content and CAT.(5)Immunity:Compared with the control group,the addition of 200 mg/kg compound probiotics significantly increased the immunoglobulin G(Ig G)content(P<0.05),the content of immunoglobulin A(IgA)were increased.(P=0.074).In summary,the compound probiotics can improve the growth performance of the growing pig and the apparent digestibility of some nutrients;it also plays an important role in enhancing antioxidant and immune immunity,and the level of compound probiotics in the growing pig diet is increased.It is advisable to add 200 mg/kg compound probiotics for growing pigs.