The Effect Of Agronomy Measures On Yield And Quality Of Notopterygium Forbesii

Notopterygium incisum is a traditional Chinese herb, and nowadays, leechdom was met by collecting natural resource almostly. The systemic study on Notopterygium incisum was scarce and the study on Agronomy Measures was blank at present. Supercritical CO2 fluid extraction is a new kind of separating echnology which is widely used in separation of natural outcomes, but used scarcely in separation of Notopterygium incisum.This paper studied the effects of Agronomy Measures and Supercritical Carbon Dioxide on yield and quality of Notopterygium forbesii, the results were as follows:1.The yield from root of Notopterygium forbesii of the no bolting treatment which was controlled increased 67.80%, the extract content increased 2.12%, and the essential oil content increased 26.19% comparing with CK. The second was getting rid of inflorescence treatment, and the last was getting rid of bolting treatment. The extract content of every treatment and CK came to the command of China Pharmacopoeia(15%), but the essential oil content did not reach the low limitation command of China Pharmacopoei(a0.028ml/g). Consequently, the no bolting treatment which was controlled was the best method considering yield and quality.2. The sequence of yield, extract content and essential oil content from Notopterygium forbesii was E> D> C> B>A in the different harvesting time of Notopterygium forbesii. The treatment E was the best harvesting time, the yield increased 367.94%,69.35%,50.36%,2.20% comparing with A,B,C,D, the extract content increased 64.25%,60.81%,30.25%,2.14% comparing with A,B,C,D, and the essential oil content increased 107.67%,86%,38.81%,8.14% comparing with A,B,C,D. The extract content of every harvesting time came to the command of Pharmacopoeia, but the essential oil content did not reach the command of Pharmacopoeia. Consequently, the treatment E was the best harvesting time.3. The different transplanting densities had significantly effect on yield, extract content and essential oil content from root of Notopterygium forbesii. The root yield descends with the decrease of the densitity, and T1 densitity was the highest yield, the yield was 3928 kg/hm2.The extract content and essential oil content from root of Notopterygium forbesii descend with the increase of the densitity, and the T6 was the highest, the extract content was 31.83%, the essential oil content was 0.0017ml/g. the extract content of every treatment came to the command of China Pharmacopoeia, but the essencial oil content did not reach the command of Pharmacopoeia. The little extract and essencial oil correspond to highest yield. So yield had negtively correlated with quality. 4. The sequence of yield, extract content and essential oil content from root of Notopterygium forbesii was N2P2>N1P1>N3P3>N4P4>N0P0. the yield of the treatment N2P2 increased 43.33% , the extract content increased 17.95%, and the essential oil content increased 43.48% comparing with CK. Different Rates of Fertilizer Application had a certain effect at weight and diameter of root from Notopterygium forbesii, and the N2P2 was the best. Each treatment had significantly effect comparing with CK at diameter of root of Notopterygium forbesii.5. In order to optimize the technology for Supercritical CO2 extracting essential oil from root of Notopterygium forbesii, the influencing factors such as extraction pressure and temperature on the yield of oil were investigated through two factors four levels experiment design. The result showed that optimum technology of extraction was as follows: extraction pressure and temperature time were 40 MPa, 45℃respectively. The yield of the oil uindes the optimum technology of extraction was about 8 times to steam distillation.6. Using the Supercritical CO2 fluid to extract the chemical compounds from root of Notopterygium forbesii, comparing fertilizer with no fertilizer component, and finding that the most chemical compounds from root of Notopterygium forbesii were Cyclopentasiloxane, Pyridine, Benzenamine and essential oil, and could be separated isomeric compound, constellation compound and trans-cis isomeric compound by Supercritical CO2 extration. Some compounds had not reported, for example, decamethyl-Cyclopentasiloxane, tripropyl- Boroxin, 3-Trifluoroacetoxy pentadecane and 1,2,3-propanetriyl ester docosanoic acid.7. The study showed that Supercritical CO2 fluid extraction had low temperature, system compactness, avoiding destroys of the small molecule and easy volatility, short extraction time, high efficience and high active compounds. There were differences of essential oils from Notopterygium forbesii between SD and SFE extraction, and Supercritical CO2 could reflect chemical compounds. So Supercritical CO2 could extract high efficience chemical compounds of Notopterygium forbesii, and was a more satisfactive extraction.