| Notopterygium is traditional chinese and Tibetan medicine.which is used by multi-ethnic.Notopterygium herbs come from the roots and rhizomes of the Umbelliferae Notopterygium Notopterygium incisumTing ex H. T. a chang and broad leaf Notopterygium, N.forbesii Bull. Herb, relieving cold Qufengchushi analgesic effect for cold cold, headache items, rheumatism, shoulder pain.Domestic and foreign high demand. However, due to the extremely difficultly the Notopterygium is cultivated, wild resources will deplete.So the commercial herbs is in short supply.In this paper,coordinating the issue"Notopterygium high altitude mountain cultivation and standardization" and"Research Notopterygium seeds (seedlings) quality standards and breeding technology point of order"and took a large number of Notopterygium cultivation of seeds and seedlings Standardization research.On the basis of the preliminary work,we carried out some of the key Notopterygium cultivation technology and quality comparative study cultivated research, guidance Notopterygium the basis to explore Notopterygium growth and development characteristics. The study results are as follows:1For the first time,nitrogen, phosphorus stress on Notopterygium underground part of the morphological changes is researched,screening promote its roots and main root development, inhibition of lateral roots and growth of suitable N, P concentration,to provide a reference for the to change physiological mechanisms reveal cultivation Notopterygium traits and improve cultivation Notopterygium traits. Middle nitrogen stress (200mg/L) and high phosphorus stress (10mg/L).And screening N,P concentration which is inhibition of the time of occurrence of adventitious roots rhizomes and promoting the increase in the total area of the oil chamber the cross-section the number of oil chamber:low nitrogen stress (100mg/L) helps to curb the time of occurrence of adventitious roots rhizomes and conducive to promoting the growth of the roots.middle nitrogen Stress (200mg/L) is the most conducive to suppress the occurrence of time and the number of adventitious roots in the roots, to promote the increase cross-section oil chamber area of the rhizome、the number of oil chamber, inhibition the occurrence of lateral roots time、to promote the number of root oil chamber、the diameter, and cross-section area;middle phosphorus stress (2mg/L) and high phosphorus stress (5mg/L) are conducive to suppress the occurrence time of adventitious roots,and help to promote the number of oil chamber in rhizome, diameter and the area of cross-section of the oil chamber.2For the first time, different degree of shade of research on Notopterygium agronomic traits, chlorophyll content and active ingredient content,we found that the degree of shade is closely related to the growth and quality of Notopterygium herbs.Provide a scientific basis for the cultivation a suitable Notopterygium the shade of choice.We used a different shade degree the biennial Notopterygium conducted test, dynamic observation found its growth and development of plants shade two layer of the basal leaves and petiole length, leaf length, leaf width were better than other treatment groups.Established Notopterygium chlorophyll content determination method,two different shade samples treated chlorophyll content were not significantly different,but there was a significant difference compared with the control group.Different shade degrees on the the biennial Notopterygium test, study its survival、long of the underground part、diameter of rhizome、long of rhizome, number of roots、fresh weight、dry weight and other economic traits,in addition,measured and calculated notopterol and Isoimperatorin content, and then the indicators weighted equally,found plants shade one layer is the shade layer suitable shading treatment, and help to improve the quality of Notopterygium.3For the first time, different seed levels with different planting densitye of research on Notopterygium agronomic traits, chlorophyll content and active ingredient content,we found seedlings levels influence growth and quality Notopterygium Significantly,and the planting density is closely related to the growth and quality of Notopterygium herbs. Provide a reference for the choice of control cultivation quality of Notopterygium seedlings and planting density.We used different seed levels with different planting densitye the biennial Notopterygium conducted test, dynamic observation found its growth and development of No.1plant of the basal leaves and petiole length, leaf length, leaf width were better than other treatment groups.Established Notopterygium chlorophyll content determination method, two different shade samples treated chlorophyll content were not significantly different, but there was a significant difference compared with the control group.Different seed levels with different planting densityeon the biennial Notopterygium test, study its surviva、long of the underground part、diameter of rhizome、long of rhizome, number of roots、fresh weight、dry weight and other economic traits,in addition,measured and calculated notopterol and Isoimperatorin content, and then the indicators weighted equally,found No.1plant is the shade layer suitable shading treatment, and help to improve the quality of Notopterygium.4This article carried out a quality research in Herbs traits, TLC identification, assay, safety and pharmacodynamic on Notopterygium of Xiaojin County, and with the wild Notopterygium of Origin were compared.Provide a reference for cultivation Notopterygium quality assessment and application.4.1In herbs traits,the difference of the cultivated Notopterygium and the wild Notopterygium is obvious, mainly from the shape, roots, root of the number, the surface color, the cross section, smell and other aspects of identification, cultivation Notopterygium No obvious roots, the roots was clusters, surface, and section lighter in color, lighter smell.4.2For the first time,carried out TLC identification research between the cultivation Notopterygium and the wild Notopterygium,established a TLC identification, notopterol and isoimperatorin as a reference substance, petroleum ether-ethyl acetate as developing solvent, launched three(once Expand8:5,10:5quadratic expansion, three expand13:5), better separation, a clear spot, Rf value is moderate. Through compared the cultivation Notopterygium and the wild Notopterygium of the thin-layer chromatography,we found that there is a significant difference, the results showed that the two chromatographic spots differences the cultivation Notopterygium in Rf value of0.92,0.76,0.53at three chromatographic spots wild Notopterygium No, Rf value0.49chromatography spots, wild Notopterygium is not obvious.4.3In the main component,in accordance with the method for the determination of Notopterygium in the current version of "Chinese Pharmacopoeia",this article studied between the cultivation Notopterygium and the wild Notopterygium, and found that both of they have reached Pharmacopoeia standard,the total amount of Notopterol and isoimperatorin of the cultivated Notopterygium with higher levels of0.7130%.Carried out experimental study on volatile oil content determination between the cultivation Notopterygium and the wild Notopterygium,he results showed that, the cultivation Notopterygium volatile oil content is1.15%,,which is not reached Pharmacopoeia standard, but the wild Notopterygium volatile oil content is1.46%,which is reached Pharmacopoeia standard.4.4For the first time.studied on the cultivation Notopterygium and the wild Notopterygium about safety and pharmacodynamic comparative, found in75%ethanol extract liquid experiment the wild Notopterygium median lethal dose LD50is the61.869g crude drug/kg, equivalent to adult clinical daily dose (0.15g/kg) of the412times, and the cultivation Notopterygium the median lethal dose LD50the is71.251g crude drug/kg, equivalent to the adult clinical daily dose (0.15g/kg)475times, indicating that in the test both of the cultivation Notopterygium and the wild Notopterygium,s security is good, and acute toxicity of cultivation Notopterygium is lower than the wild Notopterygium; anti-inflammatory test results showed that, compared with the control group, the cultivation Notopterygium high-dose group (4g/kg) and the wild Notopterygium low-dose group (2g/kg) can significantly inhibit xylene-induced ear edema in mice (p<0.05), showed significant anti-inflammatory effect; analgesic test results show that, compared with the control group, cultivation Notopterygium of the high dose group (4g/kg) can be very significantly reduced acetic acid-induced writhing frequency (p<0.01), wild Notopterygium of low-dose the group (2g/kg) can significantly reduce the vinegar induced writhing (p<0.05), cultivation Notopterygium high dose group (4g/kg) andwild Notopterygium low-dose the group (2g/kg) could significantly prolong the acetic acid-induced writhing in mice (p<0.05) showed a significant analgesic effect. |
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