Study On Rapid Propagation Of Lilium Taliense In Vitro

Lilium taliense is one of perennial bulbous flowers,belonging to the liliaceae of lilium.The whole flower droop with Anti-Vol.perianth and its color is white with Purple spots,so it is very beautiful and have high ornamental value.In this study,we use the bulblets,pedicels,flower organ etc.as the explants,which are cultured in MS medium supplemented with different hormones,such as 6-BA,NAA,2,4-D etc.we have established the Lilium taliense's regeneration system by means of callus and adventitious buds induction and studied the major factors such as explant materials, medium components and training methods which influence the induction of callus and adventitious buds.The results are as follows:(1)Using bulblets,leaves,stems,petals,receptacles,perianthes,ovaries as explants, after screening and early culture,we can see that:bulblet base and stem have the strongest renewable capacity;Leaves and petals only induced callus;Ovary directly induced adventitious buds,but in the growth of adventitious buds 15d all became glass;Receptacle and the filament without differentiation occurred.So,we celected bulblets and stems as the best induced explants.(2)Using the scale leaves as explants,we find out the best differentiation callus medium is MS+6-BA0.5mg/L+NAA0.5mg/L and MS+6-BA0.5mg/L+NAA0.1mg/L.Differentiation rates are 60.67%and 64%.Adventitious buds differentiation medium is MS+6-BA0.5mg/L+NAA0.1mg/L, and induction rate is 96.03%.To stem explants,the best medium to induce adventitious buds is MS+6-BA0.5mg/L+NAA0.05mg/L.Aseptic Node medium is MS+6-BA0.2mg/L+NAA0.05mg/L.(3)The appropriate medium for proliferation of adventitious buds is 1/2MS+NAA0.05 mg/L.The appropriate medium for roots and bulbs induction of Lilium taliense is 1/4MS+NAA0.01+refined sugar45%,regeneration plants grow well with robust bulbs,sturdy roots.The roots of regeneration plants can be induced without NAA,but the time is longer and the number of roots is limited.