Study On The Environmental - Genetic Interaction Of Tibetan And Qizhu Kashin - Related Diseases In Aba Prefecture, Sichuan Province

OBJECTIVE:This study aims to develop the specific regression model of gene-environmental interaction, qualitatify and quantitify environmental factors and genetic factors in the model by comparing the similarities and differences of the gene-environmental interaction towards the Kashin-Beck disease (KBD) between Tibetan of Ma Er Kang county and Qiang of Mao county of ABa state of Si Chuan province.METHODS:Resident’s environmental living style data collected from questionaier along with resident’s blood samples used as genetic data resources.The correlation between ABa state’s prevalence, status of level of KBD and monitoring data of its environmentwere analyzed, and linear regression modelsbetween related environmental factors prevalence and status of level of KBDwere developed and compared in Tibetan and Qiang groups.The differences of SNP genotypes of Tibetan and Qiang population were analyzed of their SNP by SHEsis; finally,The regression model of interaction between genetic and environmental risk factors on KBD was set up.RESULTS:The correlation between the environmental factors of ABa states and the prevalence and status of level of KBD shows that altitude, temperature, precipitation and selenium content in hair are significantly related with prevalence and status of level of KBD (P<0.05), furtherly, the relationship between status of level of KBD and latitude and precipitation shows linear regression (P<0.05).In Tibetan residence, advanced age, original source of water, occasionally vegetable diet, occasionally meal diet, comparatively in a poor accommodations, both parents havingof KBD, stage of ⅢinKBD of fatherand mother, all of themare risk factors for pathogenesis of Tibetan’s KBD; Female, higher education,and non farming, higher incomeand foodsprovided bygovernmentare protective factors for pathogenesis of Tibetan’s KBD. In Qiang residence,older age, poorer income, both parents carring KBD, stage of ⅢinKBD of father and mother are risk factors for pathogenesis of Qiangs KBD; higher education and foods provided by government are protective factorsfor pathogenesis of Qiang’s KBD.SNP of KBD shows, genotype AG of rs1801018 in Bcl-2 gene, AG+GG of rs1564483 in Bcl-2, and AG of rs17759659 in Bcl-2 are the risk genotypes in Tibetan’ KBD; AG+GG of rs2234767 and CT+TT of rs 1800682 in Fas are the risk genotypes in Tibetan’ KBD; CC+CAof rs6948 in caspase3 are the risk genotypes in Qiang’KBD.Interaction between genes and environmental shows, GEI of rs 1564483 in Bcle-2 gene and source of water, GEI of rs1800682 in Fas gene and frequency of vegetable diet, GEI of rs1800682 in Fas and status level of KBD in father, GEI of rs2234767 in Fas gene and status level of KBD in mother are at risk towards pathogenesis of Tibetan’s KBD. GEI of rs9648 in caspase3 gene and range of age are risk factors for Qiang’s KBD.CONCLUSION:Interaction of gene and environment factors to KBD are significantly different in Tibetan and Qiang,Tibetan’s prevalence and status of level of KBDare higher than Qiang’s may be due to there are more environmental factors interact with genetic factors in Tibetan than in Qiang.