Study On Tissue Culture Of Dendrobenthamia Capitata Var. Emeinsis

Dendrobenthamia capitata var. emeinsis Fang et Hu is a local med herb that belongs to the Cornaceae .Several natural chemical hace been isolated from Dendrobenthamia capitata var. emeinsis Fang et Hu. D. capitata var. emeinsis has been subjected to heacy collection from the wilds due to ever increasing demand .This report deals with the successful propagation of this species using in vitro techniques. The experiment with different explants of D. capitata var. emeinsis established systematically its tissue culture and plant regeneration systems. The main results were as follows:It was used in the culture that the pollution rate can bemarkedly reduced with the method of using saturated extract bleaching solution and Tween-80. Prevention and cure contrast test was conducted in order to solve the problem of severe pollution of bacteria in tissue culture for D. capitata var. emeinsis. Using sterile aqueous solution of penicillin with 4 million units per liter and 40 mins to deal with polluted seedling can effectively control the pollution of tissue culture for D. capitata var. emeinsis.It is necessary to add some activated charcoal (4g/L) into medium. The effects of activated charcoal could be attributed to providing a dark environment in the medium, adsorption of certain inhibitory substances in medium.When we use the buds as explants to induce the organgenesis in vitro, the optimal medium for inducing buds is:MS+6-BA1.0mg/L +GA1.5mg/L.Plantlets could grow healthy on this medium. The optimal medium for the multiplication of the buds is:MS+ 6-BA1.0mg/L + ZT0.5mg/L.The callus could be induced from the different explants of D. capitata var. emeinsis. The oppearance time of callus and the rate of callus induction was different was different with the different explants. The leaces and stems were beter then stem top for callus induction. The optimal medium is: MS+ NAA0.5mg/L + 6-BA1.0mg/L. It was much differently to differentiate buds from the callus. The optimal medium for the multiplication of callus is:MS+KT0.1mg/L+NAA1.0mg/L.