Detection Of Biomass By ATP Bioluminescence And Realization Of Automatic Online Monitoring

Microorganisms, which widely exist in nature, play important role in the mass exchange and transfer of energy. There is an affinity between microorganisms and human health. The research of microorganisms concerns the whole nature and human society nearly. The measurement technology for microorganism supports the research of microorganisms as a powerful tool. However, traditional measurement method takes too long time to meet with different conditions. ATP bioluminescence technology, as a rapid and convenient measurement method for microorganisms, has a great prospect in application in the fields of environmental monitoring, food hygiene, life science, medicine, etc. The research of automatic online measurement technology is innovative at home and abroad. It can solve the problems of long-term continual monitoring and measurements in the extreme environments which are not suitable for human activity. Therefore, the research of automatic online measurement technology is extremely significant.This study, based on the elementary reaction principle, analyzed different factors which influenced the ATP bioluminescence reaction, including ions and salinity, pH value, dissociative ATP, temperature and the activity of luciferase. The optimal pH value was between 7.7 and 8.0 and the temperature was about 25 ℃. The affection level of other factors was also analyzed, and solutions were proposed to eliminate the interference. Furthermore, ATP bioluminescence technology was applied in the detection of microorganisms in natural water. Research objects were waters in West Lake and marine in terms of fresh water and salt water. As results, microbialhorizontal distribution of West Lake and microbial vertical distribution of marine were obtained. Moreover, feasibility of forecast to Red Tide was studied. The result indicated that ATP bioluminescence technology, combined with automatic online measure-meter, could realize the forecast to Red Tide. Finally, as further study of the detection of microorganisms by ATP bioluminescence technology, a feasible design of automatic online measure-meter was proposed.