Priliminary Study On Total Flavonoids In Leaves And Red Pigment In Flesh Of Symplocos Paniculata (Thunb.) Miq

The paper studied the optimum extraction techniques of total flavonoids of Symplocos paniculata leaves(TFSP), the seasonal variation of TFSP content, components of TFSP, the antioxidant activities of TFSP and basic properties, stability of the red pigment in flesh. The main results were as follows:1. TFSP was extracted by using ethanol as extracting solvent and cooperating with ultrasonic wave. The primary factors which influenced extracting rate were discussed and the optimum extracting techniques were analyzed by variance analysis and orthogonal design. The results showed that the optimum extracting conditions were B3D3C2A2,1.00g sample in 50mL 60% ethanol with application of ultrasonic wave(25KHz)for 50min at 80℃. The solvent quanitity, extracting temperature, extracting time are predominant factors for extraction rate. The ultrasonic extraction is superior to reflux extraction.The content of TFSP was high in the middle ten days of April and reached the highest level in the middle ten days of August. It decreased to the lowest level in the middle ten days of December.Analysis HLPC fingerprint showed rutin, quercetin, etc. may be contained in TFSP.The scavenging effect on DPPH , the inhibitive effects of TFSP on rat in vitro liver, nephros, heart tissues and mitochondria lipid peroxidation induced by Cys- Fe2+, VitC - Fe2+, H2O2 - Fe2+ and the inhibitive effect on the hemolysis of rat RBC induced by H2O2 were studied. The results indicated that TFSP was a favorable natural antioxidant. It could scavenge DPPH ? effectively and the inhibitive effects of TFSP on lipid peroxidantion and hemolysis of rat RBC weresignificant.2 The red pigment in flesh of S. paniculata was water-soluble and alcohol-soluble anothocyanins. The stability of the pigment in acid solution was good. Light, heat, Na2SO3 K+, Ca2+, Na+, Mg2+, Al3+, Zn2+ and food additives had no negative effects on it. Hight content of glucose maltose citric acid can protect color of the pigment. H2O2, VitC, Cu2+, Mn2+, Fe3+ and hight content ofsorbic acid can degrade the pigment.