The Activities Of Total Flavonoids Extract From9Species Of Dryopteridaceae And Characterization Of Flavonoids From Dryopteris Erythrosora

Ferns of61lamilies,223genera, about2600species are ofund in China and there are about396kinds of ferns are available for medicine usage mainly in southern and southwestern region ofChina. The major bioactive compounds in ferns include flavonoids, diphenol and polyphenol，terpenoids, steroids and alkaloids. Flavonoids. the main active ingredients in medicinal efrns with astable chemical property, sinple the detection method and wide distribution in the plant, have beenlooked as important chemical ingredients of ferns. The Dryopteridaceae is a large and diverse ferniamily with a cosmopolitan distribution. About470species of13genera in Dryopteridaceae arefound in China and there are about59species of them have pharmacological activity. The studiesare mainly ofcused on the determination of total flavonoids content. However, there are littleinformation on the components and bioactivities of most fern species. The present investigationanalysises activities of total flavonoids extract from9species of Dryopteridaceae and studies thecharacterization of flavonoids from Dryopteris eiythwsova.The main results are as follows:1. The experimental materials of9species of Dryopteridaceae ferns are divided into the aerialpatr and underground part. The flavonoids content and the radical scavenging activities of50%ethanol extracts were determined. The total flavonoids content of flavonoid extracts from aerial part(Leaves and Rachis) and underground part (Roots and Stems) of9species of Dryopteridaceae fernsranged rfom1.08%to14.86%. The average flavonoids content of aerial part of Dryopteridaceaewas higher than that of underground part. The extracts from both the aerial and the undergroundparts of the9species show a better anoxidant activity. The extracts from7species show acetylcholinesterase inhibitory activity except P. tsus-simense. There is obvious positive correlationbetween the antioxidant potential and total flavonoids content. However, there are no relationshipbetween acetylcholinesterase inhibitory activity and total flavonoids content.2. Using Dryopteris erythrosora as experiment material. Flavonoids derivatives of D.erythrosora were characterized by HPLC-DAD-ESI-MS. The main lfavonoids in D. Erythrosorawere identified as gliricidin7-O-hexoside, apigenin7-0-glucoside. quercetin7-O-rutino side,quercetin7-O-galactoside. kearnpferol7-O-gentiobioside, keampefrol-3-O-rutinoside, myricetin3-O-rhamnoside and quercitrin.Then total flavonoids extract rfom D. erythrosora were extracted bydifferent organic phases. Analysis about activities of different organic phases showed that allorganic phases showed DPPH and ABTS anion scavenging activity, Reducing power, AChEinhibition activities. But the water phase was much stronger than that of other phases. Based onDPPH and ABTS anion scavenging activity，the profiles of flavonoids extracts from D. eiythrosorawater phase were seperated. Then the separate components were incorporated. Flavonoidsderivatives of separate components were characterized by HPLC-DAD-ESJ-MS.3-hydroxy-4,6,7-trimethoxyflavone, Eriodictyol were included in samplel; kaenpferoL quercetin wereincluded in sample2; there was Luteone in sample3; apigenin-7-O-rutinoside was included insample4; sample5has quercetin; quercetin, keanrpefrol, myricetin were included in sample6.