EEG Characterization In Somatoform Pain Disorder(SPD)Patients And The Mechanism Of Skeletal Muscle Aging

Background:There is a high incidence rate with very low cure rate in somatoform pain disorder(SPD).The main reasons for its poor treatment are:patients often avoid psychological problems,and more hope to explain the pain sensation with somatic lesions.Instead,doctors always emphasize that patients are psychological problems rather than organic diseases,so doctors and patients can not cooperate well.One part of the paper is to explore the EEG changes of SPD patients to provide the clinical feasibility evidence of brain function changes,the other part is to summarize the potential physical organic lesions of SPD patients as skeletal muscle aging,and preliminarily explore the mechanism of skeletal muscle aging.Part one:The mechanism of brain function changes and emotional disorders in SPD patients based on EEGObjective:To explore the changes of EEG in parietal lobe of patients with SPD in resting state and the relationship between pain and emotion in EEG response.To explore the changes of EEG in pain empathy in patients with SPD and the influence of alexithymia on empathy.Methods:18 patients with SPD and 18 healthy volunteers were included in the study.Their pain score,somatization score,anxiety and depression score,alexithymia score and interpersonal relationship index score were collected respectively.Their EEG information in resting state was collected by 10-20 system electrode method,and the preprocessed EEG signal was converted to frequency domain by DFT,The power density at specific frequency in each brain area was calculated,and the subjective scores and ERP EEG responses of patients and healthy controls were compared when they watched pictures of pain or non pain.Results:In resting state,the power density of EEG spectrum in parietal lobe increased,especially in a wave and the function connectivity of a wave in frontal-parietal lobe increased,the pain sympathy response of early N2 and late P3 and LPP in ERP decreased,and the N2 response was mediated by alexithymia.Conclusion:EEG can reflect the enlargement of somatosensory and hypersensitivity to body attention of patients with SPD.The sensory discrimination of patients with SPD is closely related to emotion.The spontaneous empathic response to other people's pain and the cognitive evaluation of other people's pain level are reduced.Part two:The effects of INK4A signaling pathway on the aging of human skeletal myoblasts and the regulation of mitochondrial membrane potentialObjective:To explore the synergism of INK4A signal pathway and mitochondrial pathway,which are two central links of cell aging.Methods:Human myoblasts were purchased and cultured.The cells were infected with p16INK4a virus vector after cultured to the third generation.The expression of p16INK4a protein was detected by WB method,and the cell senescence was identified by ?-galactosidase staining.JC-1 method was used to detect mitochondrial membrane potential.Results:After p16INK4a infected myoblasts,the morphology of lyoblasts became larger and flattened,?-galactosidase staining was positive,JC-1 staining confirmed the decrease of mitochondrial membrane potential.Conclusion:Up regulation of INK4A pathway can induce myoblast aging,and INK4a pathway and mitochondrial pathway cooperate in the process of myoblast aging.