The Study Of Subcutaneous Implantable Continuous Glucose Monitoring Micro Sensor

Diabetes as a multi-factor complex disease,is showing a high prevalence trend in our country.It sets a critical challenge to applying precision medical treatment on it.With the development and fusion of wearable device,internet and big data,continuous glucose monitoring system has become an important tool for prevention and control of diabetes.In this paper,various factors that influencing the CGMS sensing performance were studied.Synthesizing chemical deposition,electrochemical plating,and micro-electromechanical technology,a type of single needle CGM sensor,and single layer based face to face flexible CGM sensor,and a "micron-wire type" non-enzymatic glucose sensor were developed by creative electrode structure design and micro-nano technology.Following works were done in this paper:1.Focused on the basic principle of the product design and development of the CGMS,the glucose oxidase-based electrochemical glucose sensor was studied as the primary aim.The main technical issues in the current research as well as development and solutions of CGMS were listed.The new technology and application of continuous blood glucose monitoring were summarized and forecasted.2.A stainless steel based single-needle-type CGM sensor was developed,which can be directly implanted into the subcutaneous tissue without needle punch kit was constructed by surface insulate modification and microfabrication.3.The CNT/Nafion porous network which based on skeleton formed by carbon nanotube was developed by electrophoretic deposition.For the sake of electron transfer abilities,it can be used as an inner layer of electrode with mediatorless characteristic in order to avoid the oxygen dependence problem.The structure can effectively improve the electron transfer performance of the enzyme layer,enhance the long-term stability and sensitivity of the CGM sensor,and improve the selectivity.4.The outer coating membrane of CGM sensor was studied,especially the proportion of ingredients and deposition techniques.We balance the dependency of hydrophilicity and glucose mass transfer ability by modified the membrane with PEG,which play a dual role in regulating glucose penetration and enhancing biocompatibility.5.The biocompatibility of the biosensor was tested in vivo by implanting the single needle CGM sensor under rabbit skin.The results showed that the sensor has good biocompatibility.6.We designed the flexible CGM sensor which can satisfy the requirement of long-term implanting and comfortableness.A cheap,efficient and green method for surface metallization was proposed creatively,based on this work,a single layer based "back to back" flexible CGM sensor and a "micron-wire type" non-enzymatic glucose sensor was constructed.7.At last,the achievement of this paper was summarized,and we outlook the future of CGMS.