The Research On Glucose Measuring Technique Basing On Surface Plasmon Resonance

The traditional ways for glucose monitoring are realized by extracting blood from people and measuring the blood by large biochemical analyzing instrument or small blood glucose watch. Due to the pain brought by these methods, they do not adapt to continuous and frequent measurement. In addition, owing to lack of the capacity to quickly reflect the change of glucose level, the traditional way of glucose detection fails to meet the demand of application in continuous measurement. The confliction between measuring way and measuring demand becomes increasingly austere. What is more, the finding of approaches to improve the specificity and sensitivity in glucose detection is also an urgent problem that needs to be solved.In this thesis, a novel way and system are proposed to handle the problems met in glucose detection, such as low precision and poor performance in selective measurement. Basing on a miniature surface plasmon resonance sensor, we constructed an automatic measuring system. Together with this system, mechanical structure, control software, and arithmetic were also developed. Basing on this system, a large number of researches on direct and indirect measurement of glucose solution and interstitial fluid were carried through, as well as the research on experiment procedure, system performance, influence, and method to improve the system. Direct way that measures the refractive index in the surface of SPR sensor directly has succeeded in distinguish a glucose solution with the concentration of 0.1g/L, the measured refractive index of glucose solution rises linearly with the increase of concentration. By immobilizing GGBP which has specific affinity to glucose on the surface of SPR sensor, we indirectly measure the concentration of glucose solution and interstitial fluid. The detecting resolution increases to 1mg/L (G74C GGBP) and 6.25mg/L (E149C GGBP), and the SPR measuring system shows good effect in selective measurement of glucose in interstitial fluid. The experimental result reveals the bright future in applying this technique into diabetes monitoring. Besides of the automatic system, an experimental SPR system and a blue print to minimize the system are also researched.