| Objective:The experiment intends to use Chinese medicine theory and methods tobuild a new animal model of the disease-stomach empty water stop animalmodel and multi-contrast method using traditional Chinese medicine todisprove this animal model, as the objective of syndromes and after party toprovide a scientific basis for clinical use.Methods:1.The use of traditional Chinese medicine theory of the etiology, the use of alarge number of repeated gavage ice, hair plus hunger ways to build waterempty stomach to stop animal models. Kunming mice experiment,40, maleand female, weighing22-28g, were randomly divided into control group andmodel group two groups. Experimental group fed with ice six times a day,three times in the afternoon; administered to mice before each hair with fansfor15minutes after gavage with fans hair every15minutes; every morningand afternoon, fresh off once, but keep the water continuously modeling14days. Healthy control group mice were given daily routine diet. In the weekafter the completion of the establishment of animal models of animals sacrificed index detection. Determination of the amount of water, measuringthe difference between the amount of food intake, with the B-machineinspection stomach fluid retention cases, using Coomassie brilliant bluechromogenic assay antrum of nitric oxide (NO) levels using the differencebetween the amount of drinking water assay, Determination of nitratereductase in tissue homogenates NO content. Meanwhile, in strict accordancewith the determination of the protein content of the kit instructions, observethe anatomy of the stomach biopsy.2. Glycyrrhiza glabra selection of soups, Qiweibaizhu scatteredTongxieyaofang tripartite contrast to the method of treatment to disprove thedisease animal model. Modeling experiments with the first experiment tomeasure the amount of water, measuring the difference between the amount offood intake, decapitated detect blood in the blood plasma motilin, vasoactiveintestinal peptide assays using the difference between the amount of drinkingwater, processed using SPSS software statistics.3. Treatise on the use of flood tripartite (Glycyrrhiza glabra soupLingguizhugan soup, Gui Ling Gan KM) certificates on empty stomach tostop water treatment comparison model to verify the efficacy of Poria licoricesoup. Experiment with72mice were randomly divided into control group,model group, Tongxieyaofang group Qiweibaizhu scattered groups, Porialicorice soup group, domperidone group, modeling experiments with the firstexperiment, making after the mold is completed, to the animals in each groupwith the corresponding decoction (Treatise on flood tripartite decoction)gavage treatment, then measured the amount of water, measuring thedifference between the amount of food intake, decapitated blood assay usingthe difference between the amount of drinking water detected in blood plasmaand antral tissue motilin and serum gastrin antral tissue by polymerase chainreaction (PCR) measured antral tissue motilin receptor and gastrin receptor mRNA expression.4. Treatise on the use of flood tripartite (Glycyrrhiza glabra soupLingguizhugan soup, Gui Ling Gan KM) certificates on empty stomach waterretention preventive treatment, in order to understand the tripartite preventiveeffect of water on empty stomach to stop Syndrome.. Modeling method withthe first experiment, one hour before each modeling with Poria licorice soup,Lingguizhugan soup, Gui Ling Gan KM, domperidone given appropriatemedication daily irrigation by12ml/kg body weight of mice stomach until theend of modeling. Then mice were observed signs (mental status, activity, foodintake, body weight changes, urine, rat Mao Guangze degrees, stomach shape,etc.) and measuring blood motilin and gastrin content.Results:1. Observed signs and detect indicators of mice found that in the first sevendays of modeling, some mice were vomiting, hiccups, stomach slight fullness,stomach palpation auscultation with a sense of vibration of water in the first10days, nearly half of the animals vomiting, hiccups, manifestations ofspiritual malaise, chills, like a flock, decreased activity, weight loss,insensitive response to external stimuli. Within a week after the establishmentof the model, the model group were slightly dry coat, no significant change instool, individual signs of loose stools. Average daily water consumption in themodel group mice very significantly less than the control group (P <0.01),food intake model mice decreased very significantly less than the controlgroup of mice (P <0.01). Residual rate of methyl orange stomach model micevery significantly higher than the control group (P <0.01). B-show, there are alot of model mice stomach without emptying fluid retention, while the controlgroup of mice stomach is only a small amount of liquid. Antral NO contentmodel group mice very significantly higher than the control group (P <0.01). Model group compared with the control group of mice stomach fullness seemthe most obvious is the fundus area. The naked eye, cut model mice did notchange significantly after the stomach on organic, you can see no significantgastric ulcer point, no plaque erosion, mucosal white color than the controlgroup. Light microscope can be seen in the model group mice cells and gastricsquamous cell, cell proliferation significantly, between the fundus and antrumin a small amount of interstitial cells of inflammatory cell infiltration, but aslight degree, did not see the other obvious pathology changed. Fundus andantrum have not found significant organic pathology, such as gastric intestinalmetaplasia cells.2. By Poria licorice soup, Qiweibaizhu Powder, laxative side comparison ofthe treatment of pain found Poria licorice soup and water intake group wassignificantly increased compared with the model group and the other two druggroups, activity was significantly higher.5days of treatment the animals havereturned to normal state. Poria licorice decoction on mice gastric emptyingthan Qiweibaizhu scattered Tongxieyaofang, domperidone; Poria Decoctionon the role of elevated plasma motilin significantly higher Qiweibaizhuscattered groups Tongxieyaofang groups ((P <0.01or P <0.05), higher thandomperidone group, but showed no significant difference with the controlgroup, but the effect on intestinal motility has significantly reduced inhibitionof the vasoactive intestinal peptide no Qiweibaizhu scattered Tongxieyaofangsignificantly, very significantly higher than the control group (P <0.01).3. By contrast Treatise on flood tripartite treatment found that water intake andfood intake Treatise on flood tripartite group of mice than in the model groupincreased significantly (P <0.01or P <0.05), with Poria licorice soup groupmost significant increase (P <0.01). In plasma motilin aspects than the modelgroup increased significantly (P <0.01or P <0.05) motilin levels for eachtreatment group, no significant difference in motilin levels only Poria licorice soup with the control group, Ling Gui Gan dates Tang group motilin levels arevery significantly lower than the control group (P <0.01), soup groupLingguizhugan motilin levels very significantly higher than the model grouplevel but lower than the control group (P <0.05). Motilin in the antrum tissue,the show and plasma motilin similar changes. Antral gastrin in the serum andorganizational aspects of the treatment groups compared with the model groupincreased gastrin levels, elevated gastrin Poria licorice soup the mostsignificant effect. In expression of motilin receptor mRNA, compared with thecontrol group, mRNA expression of very model group decreased significantly(P <0.01); compared with the model group, mRNA expression of great floodtripartite group was significantly higher (P <0.01), Poria licorice soupincreased the most significant. In terms of gastrin receptor mRNA expression,compared with the control group, mRNA expression in model group showedsignificant increase (P <0.01), compared with the model group, mRNAexpression of Poria licorice soup is very significantly reduced, mRNAexpression Lingguizhugan soup was also significantly reduced, no statisticallysignificant difference (P>0.05) Gui Ling Gan KM mRNA expression with themodel group.4. By Treatise on empty stomach tripartite flood water retention prevention isfound in animal models, modeling, model group mice showed the first sevendays have hiccups, vomiting, stomach palpation auscultation with a sense ofvibration of water in the first10days, about half of vomiting, hiccups,manifestations of spiritual malaise, chills, gregarious clothes, decreasedactivity, weight loss, insensitive response to external stimuli, slightly dry coatcolor in mice, no significant change in stool, individual loose stools signs.After overall flood prevention treatment group were better than the tripartitemodel group. Poria licorice soup food intake and water intake group comparedwith the model group and the other two drugs was significantly increased, although the degree of mouse activity has declined, but still occasionally playwith other mice, chase, showing signs of a more active life, smooth coat stillnot playing volume, still sensitive to external stimuli, the experimenters preferfood thrown from the squirrel cage outside. In addition the overall situation inthe drug group were still two, eating and drinking is acceptable gloss coatdecline, and can still move around foraging response to outside stimuli betterthan the untreated group. Domperidone group overall poor performance,apathetic, but slightly better than the model group, mild symptoms. In additionto domperidone group, each group of mice prevention MTL levels in plasmaand tissues compared with the model group and control elevated to Porialicorice soup increased the most significant; in terms of serum gastrin, gastrintreatment groups very significantly higher than those in the untreated groupelements (P <0.01), but were lower than the control group, with Poria licoricesoup elevated gastrin most significant effect.Conclusion:In this study, the use of traditional Chinese medicine theory successfullyestablished water empty stomach to stop animal model, the model modelingstep is simple, readily available materials, laboratory equipment lessdemanding, easily controlled experimental environment, the shorter the timerequired for modeling, performance in line with gastric motility disordersobstacles stomach emptying liquid prolonged clinical features, Treatise on theflood tripartite model has a therapeutic effect and preventive effect, includingthe role of Poria licorice soup most significant, as Poria decoction of licoricewater empty stomach to stop card offers scientific basis. |
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