Expression And Function Of IER3 In Primary Hepatocarcinoma

Background:Studies indicate the immediate early response gene 3(IER3) is involved in many biological process. Recently, it is discovered that IER3 plays an important role in tumorigenesis and tumor progression. Even somebody think it’s a valuable biomarker in tumor. This study is designed to investigate the expression status and function of IER3 in primary hepatocarcinoma(PHC). Objectives: 1. To study expression of IER3 in PHC; 2. To discover the relationship between IER3 and clinicopathological features and patient survival in PHC; 3.To discover the possible mechanism of IER3. Methods: 1. Immunohistochemistry and Real-time PCR was performed to evaluate the expression levels of IER3 in 62 pathologically diagnosed human PHC specimens. 2. Immunohistochemistry was performed to evaluate the expression levels of P53, ki-67, EGFR, AFP, NF-ΚB,XIAP,Nrf2 and HO-1 in PHC. 3. The relationship between IER3 and clinicopathological features and patient survival was statistically analised with SPSS 20.0 software package. 4. The relationship between IER3 and NF-ΚB,XIAP,Nrf2 and HO-1 in PHC. Result: 1. A statistically significant association was disclosed between the expression of IER3 and P53 mutant protein(short for P53), ki-67, EGFR and the biggest diameter, differentiation grade of tumor.2. IER3 is an independent factor of PFS and OS in PHC. 3. A significant association was found between IER3 and NF-ΚB,XIAP,Nrf2 and HO-1. Conclusions:The expression level of IER3 in tumor is higher than in normal tissues. High level of IER3 expression means tumor develop quickly and poor survival. IER3 is an efficient tumor biomarker and an independent factor of PFS and OS in PHC. The role of IER3 may be performed by NF-ΚB/XIAP and Nrf2/ HO-1 signal passway.