Study On The Distribution Of HPV Genotype Prevalence In Invasive Cervical Carcinoma And Precancerous Lesions In Yangtze River Delta Area, China

Objectives:To study on the distribution of HPV genotype prevalence in invasive cervical carcinoma and precancerous lesion in Yangtze River Delta Area (Jiangzhehu Area), China to provide a scientific basis for developing prevention strategies of cervical cancer in the region.Materials and method:This was a multi-center descriptive cross-sectional study in Jiangzhehu Area, China. Four university hospitals were selected from Shanghai, Zhejiang Province and Jiangsu Province, respectively (Women’s Hospital, School of Medicine, Zhejiang University; Nanjing Drum Tower Hospital, the Affiliated Hospital of Nanjing University Medical School; The First Affiliated Hospital of Nanjing Medical University and Obstetrics and gynecology hospital of fudan university). The study population constituted women with histologically confirmed CIN1, CIN2, CIN3or invasive cervical cancer (ICC), diagnosed and treated in the four selected hospitals, ruling out those with immunodeficiency diseases including HIV, and those with previous history of cancer or CIN, during February2012to April2014. Informed consent will be secured by each subject prior to participation. The collected specimens were shipped to a central laboratory in the Shanghai research laboratory (Chaozhou Hybribio Biochemistry Ltd, Guangdong, PR China) for HPV genotyping. Analysis the distribution, age-specific and disease-specific characteristics of HPV genotype prevalence.Results: 2181qualified samples were collected,251of which were ICC and1930of which were CIN cases.200cases were detected no virus,1372cases were detected single types of infection,454cases were detected double types of infection,119cases were detected triple types of infection,29cases were detected quadruple types of infection,5cases were detected quintuple types of infection,1case was detected sextuple types of infection and1case was detected octuple types of infection. The overall average age of cervical cancer and CIN patients in Jiangzhehu area was40.93±11.8years (range,17-76years). In Jiangzhehu area, the5most common levels of HPV types were as follows:CIN1:52,58,16,33,CP; CIN2:16,58,52,33,31; CIN3:16,58,33,52,31; ICC:16,58,18,52,33. The onset age of CIN1had a sustained peak from30to40years old, which was earlier than that of CIN2, CIN3and cervical cancer. The peak age of onset of CIN2was earlier than that of CIN3and cervical cancer while the peak of the latter two appeared at the same time. There were two peaks of the onset age of cervical cancer.The first and highest peak was at40-44years old and the second peak at50-54years old.Conclusions:1. The peak ages of onset of cervical cancer in Jiangzhehu Area were40-44years old and50-54years old. The peak age of onset of CIN was30-39years old. The peak onset age of CIN was5-10years earlier than that of cervical cancer on average.2. The5most common levels of HPV types in Jiangzhehu area suggest that besides HPV16and HPV18, HPV58and HPV52showed great importance in the occurrence of cervical cancer.3. The peak onset age of the infection of high-risk HPV and that of CIN appeared at the same time which was earlier than that of cervical cancer, which corresponded to epidemiological models of viral carcinogenesis.