The Study Of HPV Genotyping Of HPV Positive Women Withcervical Cytology

[Objective]:By analyzing and summaring the examination results of HPV type testing and colposcopy biopsy pathological of cytology with no intraepithelial lesion (NILM) and human papilloma virus (HPV) positive women, and following-up the results of HPV type infection and disease progression of the women who pathological results were low grade squamous intraepithelial neoplasia (CIN Ⅰ) and Cervicitis after one year, To investigate the clinical diagnostic and follow-up management value of HPV subtype testing on cervical cytology with no intraepithelial lesion (NILM) and human papilloma virus (HPV) positive women.[Methods]:The data of 200 TCT negative and HPV positive women detected by cervical Thinprep cytologic test (TCT) and HC2-HPV-DNA testing in the outpatient department of Nanjing Drum Tower Hospital from January 2013 to December 2014 were collected, who were referred to colposcopy and histopathological examination after HPV subtype testing. The correlation between different HPV subtype infection and high-grad e cervical intraepithelial neoplasia (CIN Ⅱ+) was analyzed by using SPSS 17.0 software withusing chi square test, logistics regression statistical methods. Follow-up of the women pathologically diagnosed as CIN I and cervicitis was conducted after one year through TCT and HPV subtype testing, and the follow-up results were analyzed.[Results]:Of the 200 women,37 patients were CIN Ⅱ+(18.6%). Logistic regression analysis showed that the risk of patients with HPV 16 infection to develop CINⅡ+was 2.608 times that of patients without HPV 16 infection (OR 2.608), and the infection of other subtypes did not increase the risk of CIN Ⅱ+. Of the 163 patients with CIN I and cervicitis,151 women finished the follow-up, and 12 women were lost (all were cervicitis). The prevalence of cervical intraepithelial neoplasia in patient with homotype HPV infection was higher than that in patients with heterotype HPV infection, P<0.01. And the risk of pathological progression in patients with homotype infection was 14.89 times that in patients with heterotype HPV infection.[Conclusion]1. Cytology negative high-risk HPV positive women who had cervical combined screening have the chance of missing diagnosis of high-grade cervical intraepithelial neoplasia, HPV subtype testing can be used of managing this group of people, against HPV 16 infected patients should be promptly transferred to the diagnosis of colposcopy and the other types’ colposcopy referral can be followed by its special clinical symptoms.2. HPV typing detection can be used for the follow-up of patients who have no high cervical intraepithelial neoplasia, the patients of homotype persistent infection have high risk of pathological progression, but also more likely to occur TCT abnormalities, should be closely followed up.3. The rate of persistent infection of different types is different, the HPV 16 type is the type,of the highest,persistent infection rate and of the highest risk of pathological progression,if HPV16 persistent infection was found in follow up, should be promptly transferred to the diagnosis of colposcopy.